Document Detail


Fusion assays and models for the trophoblast.
MedLine Citation:
PMID:  18979255     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A healthy syncytium in the placenta is vital to a successful pregnancy. The trophoblast builds up the natural barrier between the mother and the developing fetus and is the site of gas, nutrition, and waste exchange. An inadequate formation of this tissue leads to several pathologies of pregnancy, which may result in fetal death during the second trimester or iatrogenic preterm delivery due to intrauterine growth restriction, preeclampsia, or abruption.Cytotrophoblastic cells fuse constantly with the overlying syncytiotrophoblast/syncytium to maintain the function of the trophoblast. Syncytin-1 is the only molecule known to directly induce fusion in the placental trophoblast. Many other proteins, such as gap junctions (e.g., connexin 40) and transcription factors, play a role in the molecular pathways directing the trophoblast turn over. Despite the significance of this process for successful placentation, the mechanisms regulating its activity remain poorly understood.In this chapter we present several different model systems that can be utilized to investigate the regulation of the cell fusion process in the trophoblast. We describe cell-based assays as well as tissue-related protocols. We show how fusion can be monitored in (1) BeWo cells as a trophoblast cell line model, (2) HEK239 using syncytin-1 as a fusion molecule, and (3) a floating villi explant model. Furthermore, we will present strategies to inhibit fusion in the different models. These techniques represent powerful tools to study the molecular mediators of cell fusion in the trophoblast.
Authors:
Sascha Drewlo; Dora Baczyk; Caroline Dunk; John Kingdom
Related Documents :
6951105 - Co-expression of mouse and rat haemoglobins in interspecific hybrids of mouse and at er...
18979235 - Yeast mating: a model system for studying cell and nuclear fusion.
20964635 - V-fusion: a convenient, nontoxic method for cell fusion.
6418635 - Complementation analysis in gaucher disease using single cell microassay techniques. ev...
12847255 - Linker for activation of t cells, zeta-associated protein-70, and src homology 2 domain...
17197195 - Using ciona to study developmental programmed cell death.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  475     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-11-03     Completed Date:  2008-11-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  363-82     Citation Subset:  IM    
Affiliation:
Womens and Infants Health, Samuel Lunenfeld Research Institute, Department of Obstetrics and Gynaecology, Mount Sinai Hospital, University of Toronto, Ontario, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biological Assay / methods*
Biotinylation
Cell Fusion / methods*
Cell Line
Cell Membrane / metabolism
Gene Products, env / metabolism
Humans
Models, Biological*
Plasmids / genetics
Pregnancy Proteins / metabolism
Transfection
Trophoblasts / cytology*
Chemical
Reg. No./Substance:
0/Gene Products, env; 0/Pregnancy Proteins; 0/syncytin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Quantitative assays for cell fusion.
Next Document:  Encapsulation of DNA and non-viral protein changes the structure of murine polyomavirus virus-like p...