| Fusion proteins for versatile antigen targeting to cell surface receptors reveal differential capacity to prime immune responses. | |
| | |
MedLine Citation:
|
PMID: 20483719 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Targeting of proteins to APCs is an attractive strategy for eliciting adaptive immune responses. However, the relationship between the choice of the targeted receptor and the quality and quantity of responses remains poorly understood. We describe a strategy for expression of Ags including hydrophobic proteins as soluble fusion proteins that are optimized for proteasome-dependent MHC class I-restricted cross-presentation and form stable complexes with a wide variety of targeting Abs. Upon s.c. immunization, these complexes were initially taken up by CD169+ lymph node subcapsular sinus macrophages. In the OVA model system, receptor-targeted antigenic complexes primed specific T and B cell responses in vitro and in vivo at least 100-fold more efficiently than Ag alone. Comparison of 10 targeting receptors allowed us to establish a ranking with respect to priming of CD8+ T cell responses and demonstrated striking differences with respect to the relative efficacy of CD8+ and CD4+ T cell subset and B cell priming. The described fusion proteins should help in developing optimized strategies for targeted delivery of protein Ags in the context of tolerization or vaccination. |
| | |
Authors:
|
Roland Kratzer; Fran?ois-Xavier Mauvais; Anne Burgevin; Emilie Barilleau; Peter van Endert |
Related Documents
:
|
10073729 - Immunization with recombinant semliki forest virus induces protection against influenza... 16618789 - T helper 1/t helper 2 cytokine imbalance in respiratory syncytial virus infection is as... 17312109 - Apoptotic cells induce tolerance by generating helpless cd8+ t cells that produce trail. 12142959 - The adaptive response of the immune system to the particular malnutrition of eating dis... 7591249 - Cyclophosphamide plus tumor necrosis factor-alpha chemoimmunotherapy cured mice: life-l... 9379479 - Cd8+ cell-mediated immune responses: relation to disease resistance and susceptibility ... 12704469 - Apoptosis, inflammatory bowel disease and carcinogenesis: overview of international and... 11704839 - The differential sensitivity of bc1-2-overexpressing human breast tumor cells to trail ... 18703789 - Il-32, a novel proinflammatory cytokine in chronic obstructive pulmonary disease. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-14 |
Journal Detail:
|
Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 184 ISSN: 1550-6606 ISO Abbreviation: J. Immunol. Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-06-07 Completed Date: 2010-06-18 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
|
Languages: eng Pagination: 6855-64 Citation Subset: AIM; IM |
Affiliation:
|
Institut National de la Sant? et de la Recherch? M?dicale, Unit? 1013, Paris, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigen Presentation / immunology* Antigens / immunology* B-Lymphocytes / immunology Blotting, Western Cross-Priming / immunology* Humans Mice Mice, Inbred C57BL Recombinant Fusion Proteins / immunology* T-Lymphocytes / immunology |
| Chemical | |
Reg. No./Substance:
|
0/Antigens; 0/Recombinant Fusion Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Lupus serum IgG induces skin inflammation through the TNFR1 signaling pathway.
Next Document: Increased hematopoietic cells in the mertk-/- mouse peritoneal cavity: a result of augmented migrati...