| Fusidic acid resistance rates and prevalence of resistance mechanisms among Staphylococcus spp. isolated in North America and Australia, 2007-2008. | |
| | |
MedLine Citation:
|
PMID: 20566766 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Among 4,167 Staphylococcus aureus and 790 coagulase-negative Staphylococcus (CoNS; not S. saprophyticus) isolates collected consecutively from North American and Australian hospitals, only 87 (1.7% overall) isolates displayed a fusidic acid (FA; also known as CEM-102) MIC of > or = 2 microg/ml (FA resistance). These strains were further evaluated with a multiplex PCR to amplify the acquired resistance genes fusB, fusC, and fusD. Mutations in fusA and fusE were evaluated in all isolates showing an absence of acquired resistance genes and/or showing FA MIC values of > or = 64 microg/ml. S. aureus resistance rates were very low in the United States (0.3%) and were higher in Canada and Australia (7.0% for both countries). Among CoNS isolates, FA resistance rates were significantly more elevated than that for S. aureus (7.2 to 20.0%; the highest rates were in Canada). All 52 (41 CoNS) FA-resistant isolates from the United States showed FA MIC results of < or = 64 microg/ml, and 7 of 11 S. aureus isolates carried fusC. CoNS strains from the United States carried fusB or fusC. In Canada, fusB and fusC occurrences were similar among S. aureus and CoNS isolates, and modestly elevated FA MIC values were observed (all MIC results were < or = 32 microg/ml). Isolates from Australia showed MIC values ranging from 2 to 32 microg/ml, and S. aureus isolates were predominantly fusC positive. fusA mutations were detected in only three S. aureus isolates, conferring FA MIC values of 2 to 8 microg/ml. Target mutations have been considered the primary FA resistance mechanism among Staphylococcus spp.; however, acquired resistance genes appear to have a dominant role in resistance against this older antimicrobial agent. In summary, this study shows that acquired genes are highly prevalent among FA-resistant strains (>90%) in three nations with distinct or absence (United States) of fusidic acid clinical use. |
| | |
Authors:
|
Mariana Castanheira; Amy A Watters; Jan M Bell; John D Turnidge; Ronald N Jones |
Related Documents
:
|
10528726 - Production of cyclopiazonic acid by molds isolated from taleggio cheese. 14573836 - Antimicrobial susceptibility of neisseria gonorrhoeae strains isolated in guangzhou, ch... 10744366 - Susceptibility of legionella species to five antibiotics and development of resistance ... 12801456 - In vitro susceptibilities of field isolates of mycoplasma agalactiae to oxytetracycline... 8586696 - Clonal relatedness of shiga-like toxin-producing escherichia coli o101 strains of human... 21596006 - An outbreak of salmonella typhimurium traced back to salami, denmark, april to june 2010. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-21 |
Journal Detail:
|
Title: Antimicrobial agents and chemotherapy Volume: 54 ISSN: 1098-6596 ISO Abbreviation: Antimicrob. Agents Chemother. Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-08-18 Completed Date: 2010-12-01 Revised Date: 2011-07-25 |
Medline Journal Info:
|
Nlm Unique ID: 0315061 Medline TA: Antimicrob Agents Chemother Country: United States |
Other Details:
|
Languages: eng Pagination: 3614-7 Citation Subset: IM |
Affiliation:
|
JMI Laboratories, 345 Beaver Kreek Centre, Suite A, North Liberty, IA 52317, USA. mariana-castanheira@jmilabs.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anti-Bacterial Agents
/
pharmacology* Australia Bacterial Proteins / genetics Drug Resistance, Bacterial / genetics Fusidic Acid / pharmacology* Microbial Sensitivity Tests Mutation North America Staphylococcus / drug effects*, genetics |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Bacterial Agents; 0/Bacterial Proteins; 6990-06-3/Fusidic Acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Clinical efficacy of combination of rifampin and streptomycin for treatment of Mycobacterium ulceran...
Next Document: In vitro effect of sulfamethoxazole-trimethoprim against Histoplasma capsulatum var. capsulatum.