Document Detail


Fused late endocytic compartments and immunostimulatory capacity of dendritic-tumor cell hybridomas.
MedLine Citation:
PMID:  19418087     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Late endocytic compartments, containing MHC class II molecules in antigen presenting cells, fuse to each other in order to deliver antigens to these molecules. We have shown previously that fusion of late endocytic compartments takes place also in hybridomas. Therefore, we investigate here whether the level of fused late endocytic compartments affects the immunostimulatory capacity of hybridomas obtained by the electrofusion of dendritic and tumor cells. The level of fused late endocytic compartments in a single hybridoma cell was assessed and samples of electrofused cells were then cocultured with autologous T cells, resulting in the priming of naïve T cells. To test the immunostimulatory capacity of hybridoma cells, T-cell-induced cytotoxicity of tumor cells was assayed. The results demonstrate that in vitro cytotoxic T cell responses are enhanced if a higher percentage of fused late endocytic compartments is present in the cell population of electrofused hybridoma cells.
Authors:
Mateja Gabrijel; Martina Bergant; Marko Kreft; Matjaz Jeras; Robert Zorec
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-06
Journal Detail:
Title:  The Journal of membrane biology     Volume:  229     ISSN:  1432-1424     ISO Abbreviation:  J. Membr. Biol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-28     Completed Date:  2009-08-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0211301     Medline TA:  J Membr Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  11-8     Citation Subset:  IM    
Affiliation:
Laboratory of Neuroendocrinology-Molecular Cell Physiology, Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia.
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MeSH Terms
Descriptor/Qualifier:
Caco-2 Cells / immunology*
Dendritic Cells / immunology*
Endocytosis / immunology*
Humans
Hybridomas / immunology*
Immunization / methods*

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