Document Detail

Fusaric acid increases melatonin levels in the weanling rat and in pineal cell cultures.
MedLine Citation:
PMID:  9055876     Owner:  NLM     Status:  MEDLINE    
Fusaric acid (FA) is produced by several Fusarium species that commonly infect cereal grains and other agricultural commodities. FA in the feed of nursing dams is lactationally transferred to the suckling offspring and alters serotonin (5-hydroxytryptamine, 5HT) in the pineal gland of the neonate rat. 5HT is involved in melatonin (MEL) production by the pineal gland. MEL is a hormone important in reproduction and seasonality in animals. Therefore, the effects of FA on MEL in the serum and pineal gland of male and female 21-d-old weanling rats from dams on an FA diet were studied. MEL was measured by enzyme-linked immunosorbent assay (ELISA), which was standardized for directly measuring MEL in rat serum and pineal homogenates. At 200 ppm in the diet of nursing dams, FA increased serum MEL in both sexes. Results obtained from ELISA were supported by high-performance liquid chromatography (HPLC) analysis with fluorescence detection. MEL analysis of the pineal gland homogenates by ELISA and HPLC supported observations in the serum. Analogously, in pineal cell monolayer cultures, FA at 1 microM and 100 microM concentrations increased MEL in a dose-dependent manner as compared to the control cells. This is the first report that FA increases MEL in vivo and in vitro and suggests that FA contamination of diets may affect mechanisms involving MEL synthesis.
A M Rimando; J K Porter
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of toxicology and environmental health     Volume:  50     ISSN:  0098-4108     ISO Abbreviation:  J Toxicol Environ Health     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-03-31     Completed Date:  1997-03-31     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7513622     Medline TA:  J Toxicol Environ Health     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  275-84     Citation Subset:  IM    
Richard B. Russell Agricultural Research Center, Toxicology and Mycotoxin Research Unit, Athens, Georgia 30613, USA.
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MeSH Terms
Cells, Cultured
Chromatography, High Pressure Liquid
Dopamine Agents / pharmacology*
Enzyme-Linked Immunosorbent Assay
Fusaric Acid / pharmacology*
Melatonin / blood,  metabolism*
Pineal Gland / drug effects*,  metabolism*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Dopamine Agents; 536-69-6/Fusaric Acid; 73-31-4/Melatonin

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