Document Detail


Fusaric acid increases melatonin levels in the weanling rat and in pineal cell cultures.
MedLine Citation:
PMID:  9055876     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fusaric acid (FA) is produced by several Fusarium species that commonly infect cereal grains and other agricultural commodities. FA in the feed of nursing dams is lactationally transferred to the suckling offspring and alters serotonin (5-hydroxytryptamine, 5HT) in the pineal gland of the neonate rat. 5HT is involved in melatonin (MEL) production by the pineal gland. MEL is a hormone important in reproduction and seasonality in animals. Therefore, the effects of FA on MEL in the serum and pineal gland of male and female 21-d-old weanling rats from dams on an FA diet were studied. MEL was measured by enzyme-linked immunosorbent assay (ELISA), which was standardized for directly measuring MEL in rat serum and pineal homogenates. At 200 ppm in the diet of nursing dams, FA increased serum MEL in both sexes. Results obtained from ELISA were supported by high-performance liquid chromatography (HPLC) analysis with fluorescence detection. MEL analysis of the pineal gland homogenates by ELISA and HPLC supported observations in the serum. Analogously, in pineal cell monolayer cultures, FA at 1 microM and 100 microM concentrations increased MEL in a dose-dependent manner as compared to the control cells. This is the first report that FA increases MEL in vivo and in vitro and suggests that FA contamination of diets may affect mechanisms involving MEL synthesis.
Authors:
A M Rimando; J K Porter
Related Documents :
25318456 - The action of peroxyl radicals, powerful deleterious reagents, explains why neither cho...
8827696 - Platelet and aorta arachidonic and eicosapentaenoic acid levels and in vitro eicosanoid...
16327286 - Influence of different dietary fat intake on liver metastasis and hepatic lipid peroxid...
15023696 - High saturated fat diet does not affect gut contractility in the rat.
1116336 - The role of plasma volume in the increase of aldosterone secretion rate during sodium d...
11101476 - Contribution of dietary protein to sulfide production in the large intestine: an in vit...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of toxicology and environmental health     Volume:  50     ISSN:  0098-4108     ISO Abbreviation:  J Toxicol Environ Health     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-03-31     Completed Date:  1997-03-31     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7513622     Medline TA:  J Toxicol Environ Health     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  275-84     Citation Subset:  IM    
Affiliation:
Richard B. Russell Agricultural Research Center, Toxicology and Mycotoxin Research Unit, Athens, Georgia 30613, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Chromatography, High Pressure Liquid
Diet
Dopamine Agents / pharmacology*
Enzyme-Linked Immunosorbent Assay
Female
Fusaric Acid / pharmacology*
Male
Melatonin / blood,  metabolism*
Pineal Gland / drug effects*,  metabolism*
Rats
Rats, Sprague-Dawley
Weaning
Chemical
Reg. No./Substance:
0/Dopamine Agents; 536-69-6/Fusaric Acid; 73-31-4/Melatonin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cancer mortality and residence near petrochemical industries in Taiwan.
Next Document:  Soluble transition metals mediate residual oil fly ash induced acute lung injury.