Document Detail

Further studies on the physico-chemical properties of Rhizopus nodosus acid lipase.
MedLine Citation:
PMID:  6735685     Owner:  NLM     Status:  MEDLINE    
The Km value of R. nodosus acid lipase was found to be 5 X 10(-2) M and 8 X 10(-3) M with olive oil and tricaprylin respectively. The lipase hydrolyzed triglycerides better than synthetic detergents and methyl esters. When synthetic triglycerides varying in fatty acid chain length were used, maximum hydrolysis was observed with tricaprylin as the substrate. Positional specificity studies indicated a preference for primary esters. The lipase was activated by albumin, NaCl and taurocholate whereas heparin had no effect. The lipase contains a single polypeptide chain with 298 amino acid residues. Glutamic acid and isoleucine were found to be the amino and carboxy-terminal residues, respectively. By gel filtration and SDS-PAGE the molecular weight was determined to be 40,000 +/- 500. The lipase was susceptible to photooxidation in the presence of methylene blue and Rose bengal whereas PMSF and thiol-group specific reagents had no appreciable effect on the lipase activity. NBS inactivated the lipase. Tryptophan residues were found to be essential for the lipase activity.
N Muthukumaran; S C Dhar
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Italian journal of biochemistry     Volume:  33     ISSN:  0021-2938     ISO Abbreviation:  Ital. J. Biochem.     Publication Date:    1984 Mar-Apr
Date Detail:
Created Date:  1984-08-15     Completed Date:  1984-08-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0376564     Medline TA:  Ital J Biochem     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  73-87     Citation Subset:  IM    
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MeSH Terms
Lipase / metabolism*
Macromolecular Substances
Methylene Blue / metabolism
Molecular Weight
Rhizopus / enzymology*
Rose Bengal / metabolism
Substrate Specificity
Reg. No./Substance:
0/Macromolecular Substances; 0/Triglycerides; 11121-48-5/Rose Bengal; 61-73-4/Methylene Blue; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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