Document Detail

Further studies on long-term preservation of rat liver: Celsior versus UW solution.
MedLine Citation:
PMID:  19180991     Owner:  NLM     Status:  MEDLINE    
The effects of Celsior solution were compared with those of the University of Wisconsin solution (UW) after 18 or 48 hours of cold storage in a perfused rat liver model. Lactate dehydrogenase (LDH), hyaluronic acid (HA) uptake, thiobarbituric acid-reactive substances (TBARS), tissue reduced (GSH) and oxidized glutathione (GSSG) and ATP were evaluated. Histochemical in situ evaluation ofLDH and reactive oxygen species (ROS) were also performed. No significant difference in LDH release, HA uptake, TBARS, ATP levels and GSH/GSSG ratio were observed between UW and Celsior solution when the livers were preserved for 18 hours. By contrast, when preservation was performed for 48 hours, LDH release, TBARS and ROS formation were higher and the ATP levels, GSH/GSSG ratio and HA uptake were lower in the liver preserved by Celsior as compared with UW. Celsior solution was as effective as UW in liver preservation up to 18 hours but the superiority of UW over Celsior solution was obtained when liver was preserved for 48 hours.
Andrea Ferrigno; Annalisa Tartaglia; Amalia Di Nucci; Vittorio Bertone; Plinio Richelmi; Daniele Neri; Isabel Freitas; Mariapia Vairetti
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vivo (Athens, Greece)     Volume:  22     ISSN:  0258-851X     ISO Abbreviation:  In Vivo     Publication Date:    2008 Nov-Dec
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-02-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806809     Medline TA:  In Vivo     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  681-6     Citation Subset:  IM    
Department of Internal Medicine, University of Pavia, Italy.
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MeSH Terms
Adenosine / pharmacology
Adenosine Triphosphate / metabolism
Allopurinol / pharmacology
Disaccharides / pharmacology
Electrolytes / pharmacology
Glutamates / pharmacology
Glutathione / metabolism,  pharmacology
Glutathione Disulfide / metabolism
Histidine / pharmacology
Insulin / pharmacology
L-Lactate Dehydrogenase / metabolism,  secretion
Liver / cytology,  drug effects,  physiology*
Mannitol / pharmacology
Organ Preservation / methods*
Organ Preservation Solutions / pharmacology*
Raffinose / pharmacology
Thiobarbituric Acid Reactive Substances / metabolism
Time Factors
Reg. No./Substance:
0/Celsior; 0/Disaccharides; 0/Electrolytes; 0/Glutamates; 0/Organ Preservation Solutions; 0/Thiobarbituric Acid Reactive Substances; 0/University of Wisconsin-lactobionate solution; 11061-68-0/Insulin; 27025-41-8/Glutathione Disulfide; 315-30-0/Allopurinol; 512-69-6/Raffinose; 56-65-5/Adenosine Triphosphate; 58-61-7/Adenosine; 69-65-8/Mannitol; 70-18-8/Glutathione; 71-00-1/Histidine; EC Dehydrogenase

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