| Further studies on the formation of choline sulfate by bacteria. | |
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MedLine Citation:
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PMID: 871964 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell extracts of Pseudomonas C12B synthesized choline sulfate (COS) from SO42-, choline chloride, and ATP. However, most of the COS-forming activity was found in culture medium supernatants of this bacterium, and that which remained with the cells was cell wall-associated. Enzyme release was independent of the carbon and (or) sulfur source used for growth and was not suppressed by increasing the divalent cation concentration of the medium. The COS-synthesizing system was inhbited in vitro by L-cysteine (greater than or equal to 10(-3) mM), SO42- (greater than 0.1 mM), and choline chloride (greater than 0.1 M). L-Cysteine (0.1-5.0 mM) did not repress the synthesis of enzymes present in the system. COS formation from SO42- in vitro was increased 2.8-fold by 10 mM adenosine 5'-phosphosulfate (APS) and 5-fold by 1 mM 3'-phosphoadenosine,5'-phosphosulfate (PAPS) during a 4-h incubation period. APS (10 mM) also inhibited the incorporation of 35SO42- into COS. Culture supernatants incubated with Na235SO4 produced two 35S-labelled metabolites having electrophoretic mobilities similar to those exhibited by authentic APS and PAPS. The synthesis of these metabolites was also inhibited in vitro by unlabelled APS and by L-cysteine. |
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Authors:
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J W Fitzgerald; P C Luschinski |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Canadian journal of microbiology Volume: 23 ISSN: 0008-4166 ISO Abbreviation: Can. J. Microbiol. Publication Date: 1977 May |
Date Detail:
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Created Date: 1977-08-25 Completed Date: 1977-08-25 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0372707 Medline TA: Can J Microbiol Country: CANADA |
Other Details:
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Languages: eng Pagination: 483-90 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine Nucleotides
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metabolism Adenosine Triphosphate / metabolism Cell-Free System Choline / biosynthesis* Cysteine / metabolism Magnesium / metabolism Phosphoadenosine Phosphosulfate / metabolism Pseudomonas / metabolism* Soil Microbiology* Sulfates / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Adenine Nucleotides; 0/Sulfates; 482-67-7/Phosphoadenosine Phosphosulfate; 52-90-4/Cysteine; 56-65-5/Adenosine Triphosphate; 62-49-7/Choline; 7439-95-4/Magnesium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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