| Further studies on amino acid transport in murine P388 leukemia cells in vitro. Presence of system y+. | |
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MedLine Citation:
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PMID: 3103685 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The transport of glycine and L-lysine into murine P388 leukemia cells has been examined. Glycine transport appears to be shared by both systems A and ASC in P388 cells. Glycine transport is Na+-dependent and is effectively blocked by alpha-(methylamino)isobutyric acid, threonine and alanine but only a marginal reduction in transport is seen with 100-fold excess cold 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid. System gly is not expressed in P388 cells. Lysine is largely transported by a Na+-independent, pH-insensitive system with a Km of 0.079 mM. Lysine transport is relatively unaffected by the addition of 100-fold excess cold alpha-(methylamino)isobutyric acid, 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid and the anionic amino acids, L-glutamate and L-aspartate. A partial inhibition of lysine transport was observed with L-threonine and L-leucine while L-arginine and L-histidine radically decreased lysine transport. Lysine appears to be transported by a system similar to the system y+ seen in cultured human fibroblasts, Ehrlich ascites cells, and hepatoma cell lines. |
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Authors:
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P Lazarus; L C Panasci |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 898 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1987 Apr |
Date Detail:
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Created Date: 1987-05-13 Completed Date: 1987-05-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 154-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism*,
pharmacology Amino Acids, Cyclic* Aminoisobutyric Acids / pharmacology Animals Biological Transport / drug effects Glycine / metabolism Hydrogen-Ion Concentration Kinetics Leukemia P388 / metabolism* Leukemia, Experimental / metabolism* Lysine / metabolism Male Mice Mice, Inbred BALB C Mice, Inbred DBA Sodium / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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R01-NSC22230/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Amino Acids, Cyclic; 0/Aminoisobutyric Acids; 20448-79-7/2-aminobicyclo(2,2,1)heptane-2-carboxylic acid; 2566-34-9/2-(methylamino)isobutyric acid; 56-40-6/Glycine; 56-87-1/Lysine; 7440-23-5/Sodium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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