Document Detail

Further studies on amino acid transport in murine P388 leukemia cells in vitro. Presence of system y+.
MedLine Citation:
PMID:  3103685     Owner:  NLM     Status:  MEDLINE    
The transport of glycine and L-lysine into murine P388 leukemia cells has been examined. Glycine transport appears to be shared by both systems A and ASC in P388 cells. Glycine transport is Na+-dependent and is effectively blocked by alpha-(methylamino)isobutyric acid, threonine and alanine but only a marginal reduction in transport is seen with 100-fold excess cold 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid. System gly is not expressed in P388 cells. Lysine is largely transported by a Na+-independent, pH-insensitive system with a Km of 0.079 mM. Lysine transport is relatively unaffected by the addition of 100-fold excess cold alpha-(methylamino)isobutyric acid, 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid and the anionic amino acids, L-glutamate and L-aspartate. A partial inhibition of lysine transport was observed with L-threonine and L-leucine while L-arginine and L-histidine radically decreased lysine transport. Lysine appears to be transported by a system similar to the system y+ seen in cultured human fibroblasts, Ehrlich ascites cells, and hepatoma cell lines.
P Lazarus; L C Panasci
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  898     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1987 Apr 
Date Detail:
Created Date:  1987-05-13     Completed Date:  1987-05-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  154-8     Citation Subset:  IM    
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MeSH Terms
Amino Acids / metabolism*,  pharmacology
Amino Acids, Cyclic*
Aminoisobutyric Acids / pharmacology
Biological Transport / drug effects
Glycine / metabolism
Hydrogen-Ion Concentration
Leukemia P388 / metabolism*
Leukemia, Experimental / metabolism*
Lysine / metabolism
Mice, Inbred BALB C
Mice, Inbred DBA
Sodium / pharmacology
Grant Support
Reg. No./Substance:
0/Amino Acids; 0/Amino Acids, Cyclic; 0/Aminoisobutyric Acids; 20448-79-7/2-aminobicyclo(2,2,1)heptane-2-carboxylic acid; 2566-34-9/2-(methylamino)isobutyric acid; 56-40-6/Glycine; 56-87-1/Lysine; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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