Document Detail


Further studies on alterations in magnesium binding during cold storage of erythrocytes.
MedLine Citation:
PMID:  3132976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Free intracellular Mg2+ concentration ([Mg2+]i) was measured in cold-stored human erythrocytes by the method of null-point titration with ionophore A23187. [Mg2+]i was 311 +/- 41 microM (mean +/- S.D.) for cells stored 0-10 days, increasing to 458 +/- 64 microM for cells stored 22-48 days. The values for stored cells were higher than those previously determined by a 31P-NMR method (Bock et al. (1985) Blood 65, 1526-1530); however, the null-point method requires extensive washing of the cells, which we have found to increase NMR-measured [Mg2+]i. The null-point values still represent a small fraction of total cell Mg2+, and confirm that binding of Mg2+ to ligands other than ATP and 2,3-bisphosphoglycerate must increase during storage. As an initial test of whether this may imply suboptimal availability of Mg2+ for cell preservation, we used A23187 to prepare erythrocytes with altered Mg2+ content, then removed ionophore and stored the cells in plasma-free medium for up to 2 weeks. Higher Mg2+ content had a very significant positive correlation (P less than 0.0001) with higher cell ATP concentrations. Storage did not significantly affect basal or Na+-stimulated efflux of Mg2+ from Mg2+-loaded red cells.
Authors:
J L Bock; Y Yusuf
Related Documents :
3595736 - A method for freezing synchronous mitotic and g1 cells.
18452386 - Cryopreservation and vitrification: recent advances in fertility preservation technolog...
19285056 - Membrane permeability coefficients of murine primary neural brain cells in the presence...
12701116 - Effects of n-acetylcysteine on dense cell formation in sickle cell disease.
19419756 - Recombinant variant fibrinogens substituted at residues gamma326cys and gamma339cys dem...
11599736 - Chloride secretion induced by thermostable direct haemolysin of vibrio parahaemolyticus...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  941     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-08-03     Completed Date:  1988-08-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  225-31     Citation Subset:  IM    
Affiliation:
Department of Pathology, New York Medical College, Valhalla.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
2,3-Diphosphoglycerate
Adenosine Triphosphate / blood
Blood Preservation
Calcimycin
Cold Temperature*
Diphosphoglyceric Acids / blood
Erythrocytes / drug effects,  metabolism*
Humans
Kinetics
Magnesium / blood*,  pharmacology
Magnetic Resonance Spectroscopy
Chemical
Reg. No./Substance:
0/Diphosphoglyceric Acids; 138-81-8/2,3-Diphosphoglycerate; 52665-69-7/Calcimycin; 56-65-5/Adenosine Triphosphate; 7439-95-4/Magnesium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of acetylcholine on electrical properties of subconfluent Madin Darby canine kidney cells.
Next Document:  Isolation, characterization and microsequence analysis of a small basic methylated DNA-binding prote...