Document Detail


Further investigations on the role of ascorbic acid in stratum corneum lipid models after UV exposure.
MedLine Citation:
PMID:  16102251     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study is the continuation of our research into vitamin C and its possible effects on human skin after topical administration. The effects of ascorbic acid, iron ions and UV irradiation on stratum corneum lipid models were investigated. The lipid models used were: a simple system (linolenic acid dispersion), a complex system (liposomes consisting of dipalmitoylphosphatidylcholine, cholesterol and linolenic acid) and complex systems with additionally incorporated ceramides (types III and IV). The lipid peroxidation was quantified by the thiobarbituric acid assay. A human adult low-calcium high-temperature (HaCaT) keratinocytes cell culture was used as a second in-vitro model. The amount of intracellular peroxides was determined by measuring the fluorescence intensity using the dihydrorhodamine 123 assay. Electron paramagnetic resonance spectroscopy was used to study the influence of ascorbic acid and iron ions on the signal intensity of 5-doxylstearic acid during UV exposure. Ascorbic acid showed prooxidative properties in the thiobarbituric acid assay whereas cell protection was measured in the HaCaT keratinocytes experiments. Electron paramagnetic resonance investigations revealed different extents of free radical production generated by iron ions, ascorbic acid and UV irradiation. In evaluating the results from this study new aspects of the mechanism of lipid damage caused by these three factors were suggested, transcending the simple redox behaviour of ascorbic acid.
Authors:
Hagen Trommer; Rolf Böttcher; Christoph Huschka; Wolfgang Wohlrab; Reinhard H H Neubert
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of pharmacy and pharmacology     Volume:  57     ISSN:  0022-3573     ISO Abbreviation:  J. Pharm. Pharmacol.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-16     Completed Date:  2006-09-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376363     Medline TA:  J Pharm Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  963-72     Citation Subset:  IM    
Affiliation:
Martin-Luther-University Halle-Wittenberg, School of Pharmacy, Institute of Pharmaceutics and Biopharmaceutics, Wolfgang-Langenbeck-Strasse 4, D-06120 Halle (Saale), Germany. trommer@pharmazie.uni-halle.de
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MeSH Terms
Descriptor/Qualifier:
Ascorbic Acid / pharmacology*
Cell Line
Ceramides / chemistry,  metabolism,  radiation effects
Cholesterol / chemistry,  metabolism,  radiation effects
Electron Spin Resonance Spectroscopy
Ferrous Compounds
Humans
Keratinocytes
Linoleic Acid / chemistry,  metabolism,  radiation effects
Lipid Peroxidation
Liposomes
Membrane Lipids / chemistry,  metabolism*
Reactive Oxygen Species / metabolism
Rhodamines
Skin / drug effects*,  metabolism,  radiation effects*
Ultraviolet Rays*
Chemical
Reg. No./Substance:
0/Ceramides; 0/Ferrous Compounds; 0/Liposomes; 0/Membrane Lipids; 0/Reactive Oxygen Species; 0/Rhodamines; 109244-58-8/dihydrorhodamine 123; 2197-37-7/Linoleic Acid; 50-81-7/Ascorbic Acid; 57-88-5/Cholesterol; 7720-78-7/ferrous sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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