Document Detail

Further characterization of rat dihydroceramide desaturase: tissue distribution, subcellular localization, and substrate specificity.
MedLine Citation:
PMID:  11104018     Owner:  NLM     Status:  MEDLINE    
The introduction of the double bond in the sphingoid backbone of sphingolipids occurs at the level of dihydroceramide via an NADPH-dependent desaturase, as discovered in permeabilized rat hepatocytes. In the rat, the enzyme activity, which has now been further characterized, appeared to be mostly enriched in liver and Harderian gland. By means of subcellular fractionation of rat liver homogenates and density gradient separation of microsomal fractions, the desaturase was localized to the endoplasmic reticulum. Various detergents were inhibitory to the enzyme, and maximal activities were obtained in the presence of NADPH and when the substrate was complexed to albumin. In the presence of albumin, the chain length of the fatty acid of the truncated dihydroceramides hardly affected the activity. Finally, in view of a likely evolutionary relationship between desaturases and hydroxylases, the formation of hydroxylated intermediates was analyzed. No evidence for their presence was found under our assay conditions.
C Causeret; L Geeraert; G Van der Hoeven; G P Mannaerts; P P Van Veldhoven
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  35     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2001-02-05     Completed Date:  2001-03-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1117-25     Citation Subset:  IM    
Katholieke Universiteit Leuven, Faculteit Geneeskunde, Departement Moleculaire Celbiologie, Belgium.
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MeSH Terms
Albumins / metabolism
Centrifugation, Density Gradient
Ceramides / biosynthesis,  chemistry
Detergents / pharmacology
Endoplasmic Reticulum / metabolism
Harderian Gland / metabolism
Liver / metabolism
Microsomes, Liver / metabolism
Models, Chemical
NADP / metabolism
Oxidoreductases / chemistry*,  pharmacokinetics*
Rats, Wistar
Subcellular Fractions / metabolism
Substrate Specificity
Tissue Distribution
Reg. No./Substance:
0/Albumins; 0/Ceramides; 0/Detergents; 53-59-8/NADP; EC 1.-/Oxidoreductases; EC 1.3.1.-/dihydroceramide desaturase

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