Document Detail


Further characterization of the genotoxicity of formaldehyde in vitro by the sister chromatid exchange test and co-cultivation experiments.
MedLine Citation:
PMID:  18477654     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The induction of sister chromatid exchanges (SCE) was used to further characterize the genotoxic action of formaldehyde (FA) on cultured mammalian cells. FA induced SCE in V79 Chinese hamster cells and A549 human lung cells in a concentration-related manner. Addition of 5-bromodeoxyuridine (BrdUrd) for the differentiation of sister chromatids to visualize SCE 4 h after the FA treatment led to a clearly reduced induction of SCE in agreement with the repair kinetics of FA-induced DNA-protein cross-links. When A549 cells were treated with FA for 1 h and then co-cultivated with V79 cells in the presence of BrdUrd, a clear induction of SCE was measured in V79 cells. When the same experiment was performed including washing and change of medium after the FA treatment, no induction of SCE was measured in V79 cells. These results indicate that reactive FA remains in the cell culture medium for a longer time period despite the high reactivity of FA with macromolecules. However, FA that has entered a cell is not released and does not damage other cells. Possible implications for the mutagenicity of FA in vivo will be discussed.
Authors:
Simone Neuss; Günter Speit
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-13
Journal Detail:
Title:  Mutagenesis     Volume:  23     ISSN:  1464-3804     ISO Abbreviation:  Mutagenesis     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-01     Completed Date:  2008-10-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8707812     Medline TA:  Mutagenesis     Country:  England    
Other Details:
Languages:  eng     Pagination:  355-7     Citation Subset:  IM    
Affiliation:
Institut für Humangenetik, Universität Ulm, D-89069 Ulm, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Coculture Techniques
Cricetinae
Cricetulus
DNA Damage*
Formaldehyde / toxicity*
Humans
Occupational Exposure
Sister Chromatid Exchange / drug effects*
Chemical
Reg. No./Substance:
50-00-0/Formaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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