Document Detail

Further characterization of a broad-spectrum antiviral substance in human serum.
MedLine Citation:
PMID:  1335259     Owner:  NLM     Status:  MEDLINE    
A broadly active antiviral glycoprotein (UTI beta) occurs naturally in human sera at an average antiviral titer of 50 U/ml. This inhibitor is active against all virus families tested to date, including representative poxviruses, herpesviruses, enteroviruses, paramyxoviruses, alpha-viruses, flaviviruses, bunyaviruses, and rhabdoviruses. It is a glycoprotein of approximately 60,000 +/- 10,000 Da, which is stable at pH 2 to 10 and at 80 degrees C for up to 10 min. Mild oxidation with NaIO4 and treatment with glycosidases inactivates the material. Proteolytic degradation of the inhibitor molecule releases small active components of < 1000 Da, which retain antiviral activity. This activity of the small components has increased heat stability (120 degrees C for 15 min) and is inactivated by glycosidases. The antiviral activity thus appears to reside mainly in the oligosaccharide moiety of the glycoprotein. The inhibitor does not neutralize virions, but prevents attachment of most viruses to cells. These properties occur also in highly purified preparations. These findings indicate that human serum contains significant concentrations of a broadly active antiviral glycoprotein, which is distinct from interferon and other antiviral substances naturally found in human body fluids and tissues.
I P Singh; D H Coppenhaver; A K Chopra; S Baron
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Viral immunology     Volume:  5     ISSN:  0882-8245     ISO Abbreviation:  Viral Immunol.     Publication Date:  1992  
Date Detail:
Created Date:  1993-02-01     Completed Date:  1993-02-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8801552     Medline TA:  Viral Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  293-303     Citation Subset:  IM    
Department of Microbiology, University of Texas Medical Branch, Galveston.
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MeSH Terms
Antiviral Agents / blood*,  chemistry
Endopeptidases / pharmacology
Glycoside Hydrolases / pharmacology
Hot Temperature
Middle Aged
Molecular Weight
Periodic Acid / chemistry
Reg. No./Substance:
0/Antiviral Agents; 10450-60-9/Periodic Acid; EC 3.2.1.-/Glycoside Hydrolases; EC 3.4.-/Endopeptidases

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