Document Detail


Further characterization of Staphylococcus epidermidis transposon mutants deficient in primary attachment or intercellular adhesion.
MedLine Citation:
PMID:  9532266     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biofilm formation is suggested to be the result of primary attachment of Staphylococcus epidermidis cells to a polymer surface followed by accumulation in multilayered cell clusters. Here we describe the further characterization of transposon (Tn917) mutants of Staphylococcus epidermidis O-47 having been biofilm-negative in a polystyrene microtiter plate adhesion assay. Among 5000 Tn917 insertion strains, we isolated four biofilm-negative mutants, each carrying one copy of Tn917. The mutants could be divided into two phenotypic classes: class A (mut1 and mut1a) and class B (mut2 and mut2a). Mutants of phenotype class A lacked four cell surface proteins and were affected in the primary attachment to polystyrene, but remained able to form multilayered cell clusters and to produce PIA. Mutants of phenotype class B were able to attach to polystyrene, but did not form multilayered cell clusters nor produce PIA. The cell surface protein pattern relative to the wild type was unchanged in class B mutants. On Congo red agar, the wild type and class A mutants formed black colonies (positive reaction on Congo red agar) while class B mutant colonies were red (negative reaction). The initial binding of cells to polystyrene and the ability to form multilayered cell clusters were found to be phenotypically and genetically distinct traits.
Authors:
C Heilmann; F Götz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zentralblatt für Bakteriologie : international journal of medical microbiology     Volume:  287     ISSN:  0934-8840     ISO Abbreviation:  Zentralbl. Bakteriol.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-04-07     Completed Date:  1998-04-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9203851     Medline TA:  Zentralbl Bakteriol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  69-83     Citation Subset:  IM; S    
Affiliation:
Mikrobielle Genetik, Universität Tübingen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adhesins, Bacterial / isolation & purification
Bacterial Adhesion / genetics,  physiology
Biofilms / growth & development*
Blotting, Southern
DNA Transposable Elements / genetics*
Humans
Microscopy, Fluorescence
Phenotype
Staphylococcus epidermidis / genetics,  physiology*
Chemical
Reg. No./Substance:
0/Adhesins, Bacterial; 0/DNA Transposable Elements

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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