Document Detail

Further characterization of Staphylococcus epidermidis transposon mutants deficient in primary attachment or intercellular adhesion.
MedLine Citation:
PMID:  9532266     Owner:  NLM     Status:  MEDLINE    
Biofilm formation is suggested to be the result of primary attachment of Staphylococcus epidermidis cells to a polymer surface followed by accumulation in multilayered cell clusters. Here we describe the further characterization of transposon (Tn917) mutants of Staphylococcus epidermidis O-47 having been biofilm-negative in a polystyrene microtiter plate adhesion assay. Among 5000 Tn917 insertion strains, we isolated four biofilm-negative mutants, each carrying one copy of Tn917. The mutants could be divided into two phenotypic classes: class A (mut1 and mut1a) and class B (mut2 and mut2a). Mutants of phenotype class A lacked four cell surface proteins and were affected in the primary attachment to polystyrene, but remained able to form multilayered cell clusters and to produce PIA. Mutants of phenotype class B were able to attach to polystyrene, but did not form multilayered cell clusters nor produce PIA. The cell surface protein pattern relative to the wild type was unchanged in class B mutants. On Congo red agar, the wild type and class A mutants formed black colonies (positive reaction on Congo red agar) while class B mutant colonies were red (negative reaction). The initial binding of cells to polystyrene and the ability to form multilayered cell clusters were found to be phenotypically and genetically distinct traits.
C Heilmann; F Götz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zentralblatt für Bakteriologie : international journal of medical microbiology     Volume:  287     ISSN:  0934-8840     ISO Abbreviation:  Zentralbl. Bakteriol.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-04-07     Completed Date:  1998-04-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9203851     Medline TA:  Zentralbl Bakteriol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  69-83     Citation Subset:  IM; S    
Mikrobielle Genetik, Universität Tübingen, Germany.
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MeSH Terms
Adhesins, Bacterial / isolation & purification
Bacterial Adhesion / genetics,  physiology
Biofilms / growth & development*
Blotting, Southern
DNA Transposable Elements / genetics*
Microscopy, Fluorescence
Staphylococcus epidermidis / genetics,  physiology*
Reg. No./Substance:
0/Adhesins, Bacterial; 0/DNA Transposable Elements

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