Document Detail


Further characterization of BC3H1 myogenic cells reveals lack of p53 activity and underexpression of several p53 regulated and extracellular matrix-associated gene products.
MedLine Citation:
PMID:  12534338     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To catalog factors that may contribute to the completion of myogenesis, we have been looking for molecular differences between BC3H1 and C2C12 cells. Cells of the BC3H1 tumor line, though myogenic, are nonfusing, and withdraw from the cell cycle only reversibly, whereas cells of the C2C12 line fuse, differentiate terminally, and express several muscle-specific gene products that BC3H1 cells do not. Relative to C2C12 cells, BC3H1 cells underaccumulated cyclin-dependent kinase inhibitor p21 and underaccumulated transcripts for p21, GADD45, CDO, decorin, osteopontin, H19, fibronectin, and thrombospondin-1 (tsp-1). Levels of accumulation of H19, tsp-1, and larger isoforms of fibronectin messenger ribonucleic acid (mRNA) were found to increase in response to expression of myogenic regulatory factors as shown by their accumulation in differentiated myogenically converted 10T1/2 cells but not in 10T1/2 fibroblasts. BC3H1s accumulated a temperature-insensitive, geldanamycin-sensitive, misfolded form of p53 incapable of transactivating a p53 responsive reporter, consistent with underexpression of p21, GADD45, and tsp-1. BC3H1 and C2C12 cells were similar with respect to upregulation of p27 protein, downregulation of mitogen-activated protein kinase phosphatase-1 (MKP-1) protein, upregulation of retinoblastoma (Rb) mRNA, and nuclear localization of hypophosphorylated Rb. Cells of both lines expressed the muscle-specific 1b isoform of MEF2D. Although nonfusing in the short term, after more than 18 d in differentiation medium, some cultures of BC3H1 cells formed viable multinucleated cells in which the nuclei did not reinitiate synthesis of DNA in response to serum. Our findings suggest participation of tsp-1 and specific isoforms of fibronectin in myogenesis and suggest additional avenues of research in myogenesis and oncogenesis.
Authors:
Sandra B Sharp; Maria Villalvazo; Mickey Huang; Rodolfo Gonzalez; Irania Alarcon; Matthew Bahamonde; Diane M D'Agostin; Sagar Damle; Alex Espinosa; Seog J Han; Jessica Liu; Paula Navarro; Hugo Salguero; Jina Son; Son Vu
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  38     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:    2002 Jul-Aug
Date Detail:
Created Date:  2003-01-21     Completed Date:  2004-01-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  United States    
Other Details:
Languages:  eng     Pagination:  382-93     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, California State University, 5151 State University Drive, Los Angeles, California 90032, USA. ssharp@calstatela.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
Cell Line
Extracellular Matrix Proteins / genetics,  metabolism
Gene Expression Regulation*
Mice
Muscle Proteins / genetics,  metabolism
Muscles / cytology,  metabolism
RNA, Messenger / genetics,  metabolism
Tumor Suppressor Protein p53 / metabolism*
Grant Support
ID/Acronym/Agency:
GM61331/GM/NIGMS NIH HHS; S06GM08101/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Extracellular Matrix Proteins; 0/Muscle Proteins; 0/RNA, Messenger; 0/Tumor Suppressor Protein p53

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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