Document Detail


Further characterization of Ascaris suum component(s) with suppressive activity on the IgE antibody response.
MedLine Citation:
PMID:  2101078     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to characterize the component(s) of Ascaris suum responsible for damping of the IgE antibody production we demonstrated that the extract incubated in sodium acetate buffer, pH 4.5, maintained its suppressive effect and the same protein banding pattern by SDS-PAGE. Elimination of the lipoprotein components of the extract also left its damping properties unchanged. SDS-PAGE of the lipoprotein-free extract revealed practically the same pattern as shown by the whole extract, except for the high molecular weight polypeptides. These results indicate that the suppressive component(s) of A. suum did not precipitate and retained their activity at low pH. In addition, they appear not to be lipoproteins.
Authors:
M F Soares; A Perini; I Mota; M S Macedo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]     Volume:  23     ISSN:  0100-879X     ISO Abbreviation:  Braz. J. Med. Biol. Res.     Publication Date:  1990  
Date Detail:
Created Date:  1991-09-05     Completed Date:  1991-09-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8112917     Medline TA:  Braz J Med Biol Res     Country:  BRAZIL    
Other Details:
Languages:  eng     Pagination:  589-92     Citation Subset:  IM    
Affiliation:
Centro de Formação e Pesquisa em Imunologia, Instituto Butantan, São Paulo, Brasil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Helminth / biosynthesis*
Ascaris / immunology*
Electrophoresis, Polyacrylamide Gel
Immune Tolerance
Immunoglobulin E / biosynthesis*
Mice
Mice, Inbred A
Ovalbumin / immunology
Chemical
Reg. No./Substance:
0/Antibodies, Helminth; 37341-29-0/Immunoglobulin E; 9006-59-1/Ovalbumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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