Document Detail


Further biochemical and biophysical characterisation of scyllin, Scylla serrata hemolymph lectin.
MedLine Citation:
PMID:  9192099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The amino acid and carbohydrate analysis of scyllin, a low molecular weight lectin purified from Scylla serrata (edible crab) haemolymph reveal that scyllin is rich in acidic and neutral amino acids and contains high amount of mannose. UV absorption of scyllin is perturbed by DMSO at 272 nm showing the presence of tryptophan molecule in scyllin exposed and accessible to the solvent. The oxidation of tryptophan molecule by N-bromosuccinimide results in loss of haemagglutinating activity of lectin. The study of thermodynamic parameters of scyllin-glycoproteins interaction suggests that ceruloplasmin is the most potent inhibitors of scyllin of all the glycoproteins studied.
Authors:
T Chattopadhyay; B Chatterjee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry and molecular biology international     Volume:  42     ISSN:  1039-9712     ISO Abbreviation:  Biochem. Mol. Biol. Int.     Publication Date:  1997 Jun 
Date Detail:
Created Date:  1997-08-04     Completed Date:  1997-08-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9306673     Medline TA:  Biochem Mol Biol Int     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  183-91     Citation Subset:  IM    
Affiliation:
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Calcutta, India.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Bromosuccinimide / chemistry
Carbohydrates / analysis
Ceruloplasmin / pharmacology
Dimethyl Sulfoxide / pharmacology
Hemagglutination
Hemolymph / chemistry*
Hydrogen-Ion Concentration
Lectins / analysis,  antagonists & inhibitors,  chemistry*
Mannose / analysis
Molecular Weight
Oxidation-Reduction
Spectrophotometry, Ultraviolet
Thermodynamics
Tryptophan / analysis
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Lectins; 31103-86-3/Mannose; 39660-53-2/Bromosuccinimide; 67-68-5/Dimethyl Sulfoxide; 73-22-3/Tryptophan; EC 1.16.3.1/Ceruloplasmin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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