Document Detail


Further analysis of lethal factors in cycloheximide-treated mice given low doses of bacterial lipopolysaccharide.
MedLine Citation:
PMID:  2715398     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endotoxin challenge (0.2 micrograms per mouse) 6 h after a standard dose of cycloheximide was compared with the previously-reported effects of simultaneous injection of cycloheximide and endotoxin. With the submicrogram dose of endotoxin given 6 h after cycloheximide, fatalities occurred without evidence of thrombogenic bilateral renal cortical necrosis which characterized mice dying after the two agents were given together. Anticoagulation with heparin or with ancrod is life-saving, indicating that cycloheximide-treated mice were fatally susceptible to fibrinogen-to-fibrin conversion, and that, in contrast to the situation where cycloheximide and endotoxin are given simultaneously, there was no essential demand for supplementary glycocorticosteroid. A dose of 5.0 micrograms of endotoxin given 6 h after cycloheximide was fatal; again no renal cortical necrosis occurred, but both ancrod and hydrocortisone were essential to ensure survival.
Authors:
E W Parry
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of comparative pathology     Volume:  100     ISSN:  0021-9975     ISO Abbreviation:  J. Comp. Pathol.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-06-19     Completed Date:  1989-06-19     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0102444     Medline TA:  J Comp Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  155-60     Citation Subset:  IM    
Affiliation:
Department of Human Anatomy and Cell Biology, The University, Liverpool, U.K.
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MeSH Terms
Descriptor/Qualifier:
Ancrod / therapeutic use
Animals
Cycloheximide / pharmacology*
Endotoxins / toxicity
Heparin / therapeutic use
Hydrocortisone / therapeutic use
Lipopolysaccharides / toxicity*
Male
Mice
Mice, Inbred CBA
Time Factors
Chemical
Reg. No./Substance:
0/Endotoxins; 0/Lipopolysaccharides; 50-23-7/Hydrocortisone; 66-81-9/Cycloheximide; 9005-49-6/Heparin; EC 3.4.21.-/Ancrod

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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