| Furosemide stimulates K transport in HCD57 erythroid cells. | |
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MedLine Citation:
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PMID: 10833533 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We examined the influence of serum and furosemide on K movement and cell volume in HCD57 cells, a murine erythroleukemia cell line, which require erythropoietin (EPO) for survival. We found that maintenance of cell volume depends on the concentration of serum in the culture medium. In isotonic medium containing 20% serum, HCD57 cells maintain their steady-state volume. In contrast, the cells shrink progressively as medium serum content is reduced. In serum-free medium, raising external K to 75 mm prevents cell shrinkage and a further increase in K to 145 mm results in swelling, revealing a role for K permeability in the regulation of cell volume. Of particular interest has been a serendipitous finding with furosemide. Below an external K concentration of 2.1 +/- 0.3 mm in medium containing 2% serum, furosemide inhibits K uptake, probably stemming from its well known inhibitory action on KCl cotransport. However, above that K concentration, furosemide stimulates K uptake in a dose-dependent manner. Moreover, furosemide potentiates cell shrinkage induced by serum withdrawal. These findings suggest that the transport machinery mediating cellular shrinkage, once primed by serum depletion, becomes receptive to a second stimulus. |
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Authors:
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H D Kim; J T Turner; J E Burnett; N S Joo; B H Han; R W Lim; J W Bowen; R G Kirk; P Lee |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of membrane biology Volume: 175 ISSN: 0022-2631 ISO Abbreviation: J. Membr. Biol. Publication Date: 2000 Jun |
Date Detail:
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Created Date: 2000-08-18 Completed Date: 2000-08-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0211301 Medline TA: J Membr Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 235-44 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of Missouri, M517B Medical Sciences Building, One Hospital Center Dr., Columbia, MO 65212, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport / drug effects Carrier Proteins / genetics Chlorides / metabolism Culture Media / pharmacology Dose-Response Relationship, Drug Furosemide / pharmacology* Leukemia, Erythroblastic, Acute Mice Potassium / metabolism* Rats Serum Albumin, Bovine / pharmacology Symporters* Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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DE07389/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Chlorides; 0/Culture Media; 0/Serum Albumin, Bovine; 0/Symporters; 0/potassium-chloride symporters; 54-31-9/Furosemide; 7440-09-7/Potassium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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