Document Detail


Furin is important but not essential for the proteolytic maturation of gp160 of HIV-1.
MedLine Citation:
PMID:  7774724     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The envelope glycoproteins of HIV are required for viral infectivity. Proteolysis of the precursor envelope glycoprotein gp160 results in the formation of gp120 and gp41. Cleavage occurs after the sequence Arg-Glu-Lys-Arg. This sequence is expected to be a substrate for the cellular protease furin. We examined whether furin is responsible for cleavage of gp160 by using a furin-deficient CHO cell line and the same cell line transfected with furin cDNA. Data obtained from viral transmission assays suggested that furin increased viral infectivity but was not essential for the maturation of gp160, implying that other proprotein processing enzymes also recognize this putative furin cleavage site.
Authors:
M Gu; J Rappaport; S H Leppla
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  FEBS letters     Volume:  365     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-07-10     Completed Date:  1995-07-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  95-7     Citation Subset:  IM; X    
Affiliation:
Laboratory of Microbial Ecology, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
CHO Cells
Cricetinae
Furin
Gene Products, env / metabolism*
HIV Core Protein p24 / analysis
HIV Envelope Protein gp160
HIV-1 / metabolism*
Humans
Mice
Molecular Sequence Data
Polymerase Chain Reaction
Protein Precursors / metabolism*
Protein Processing, Post-Translational*
Recombinant Proteins / metabolism
Subtilisins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Gene Products, env; 0/HIV Core Protein p24; 0/HIV Envelope Protein gp160; 0/Protein Precursors; 0/Recombinant Proteins; EC 3.4.21.-/Subtilisins; EC 3.4.21.75/Furin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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