Document Detail


Fungicidal and cytotoxic activity of a Capsicum chinense defensin expressed by endothelial cells.
MedLine Citation:
PMID:  16794772     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plant defensins are antimicrobial peptides that exhibit mainly antifungal activity against a broad range of plant fungal pathogens. However, their actions against Candida albicans have not been extensively studied. The mRNA for gamma-thionin, a defensin from Capsicum chinense, has been expressed in bovine endothelial cells. The conditioned medium of these cells showed antifungal activity on germ tube formation (60-70% of inhibition) and on the viability of C. albicans (70-80% of inhibition). Additionally, C. albicans was not able to penetrate transfected cells. Conditioned medium from these cells also inhibited the viability (80%) of the human tumor cell line, HeLa.
Authors:
José L Anaya-López; Joel E López-Meza; Victor M Baizabal-Aguirre; Horacio Cano-Camacho; Alejandra Ochoa-Zarzosa
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-23
Journal Detail:
Title:  Biotechnology letters     Volume:  28     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-06     Completed Date:  2007-02-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1101-8     Citation Subset:  IM    
Affiliation:
Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, México.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antifungal Agents / metabolism,  pharmacology
Antineoplastic Agents / metabolism,  pharmacology
Candida albicans / cytology,  drug effects*
Capsicum / genetics,  metabolism*
Cattle
Cell Survival / drug effects*
Cells, Cultured
Defensins / genetics,  metabolism*,  pharmacology*
Dose-Response Relationship, Drug
Endothelial Cells / metabolism*
Hela Cells
Humans
Protein Engineering / methods
Recombinant Proteins / metabolism,  pharmacology
Chemical
Reg. No./Substance:
0/Antifungal Agents; 0/Antineoplastic Agents; 0/Defensins; 0/Recombinant Proteins

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