| Fungicidal and cytotoxic activity of a Capsicum chinense defensin expressed by endothelial cells. | |
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MedLine Citation:
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PMID: 16794772 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plant defensins are antimicrobial peptides that exhibit mainly antifungal activity against a broad range of plant fungal pathogens. However, their actions against Candida albicans have not been extensively studied. The mRNA for gamma-thionin, a defensin from Capsicum chinense, has been expressed in bovine endothelial cells. The conditioned medium of these cells showed antifungal activity on germ tube formation (60-70% of inhibition) and on the viability of C. albicans (70-80% of inhibition). Additionally, C. albicans was not able to penetrate transfected cells. Conditioned medium from these cells also inhibited the viability (80%) of the human tumor cell line, HeLa. |
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Authors:
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José L Anaya-López; Joel E López-Meza; Victor M Baizabal-Aguirre; Horacio Cano-Camacho; Alejandra Ochoa-Zarzosa |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-06-23 |
Journal Detail:
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Title: Biotechnology letters Volume: 28 ISSN: 0141-5492 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-07-06 Completed Date: 2007-02-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1101-8 Citation Subset: IM |
Affiliation:
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Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, México. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antifungal Agents / metabolism, pharmacology Antineoplastic Agents / metabolism, pharmacology Candida albicans / cytology, drug effects* Capsicum / genetics, metabolism* Cattle Cell Survival / drug effects* Cells, Cultured Defensins / genetics, metabolism*, pharmacology* Dose-Response Relationship, Drug Endothelial Cells / metabolism* Hela Cells Humans Protein Engineering / methods Recombinant Proteins / metabolism, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antifungal Agents; 0/Antineoplastic Agents; 0/Defensins; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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