Document Detail


Fungal mycelia show lag time before re-growth on endogenous carbon.
MedLine Citation:
PMID:  18183629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nutrient starvation is a common occurrence for filamentous fungi. To better understand the effects of starvation, we used a parallel plate flow chamber to study individual fungal mycelia when subjected to a step change in glucose concentration. We report the presence of a finite "lag time" in starved mycelia during which they ceased to grow/extend while switching from growth on exogenous carbon to re-growth on endogenous carbon. This lag time precedes other morphological or physiological changes such as change in growth rate (50-70% reduction), vacuolation (up to 16%), and decreased hyphal diameter (almost 50% reduction). Data suggests that during lag time, vacuolar degradation produces sufficient endogenous carbon to support survival and restart hyphal extension. Lag time is inversely related to the size of the mycelium at the time of starvation, which suggests a critical flow of endogenous carbon to the apical tip. We present a mathematical model consistent with our experimental observations that relate lag time, area, and flow of endogenous carbon.
Authors:
Judith K Pollack; Zheng Jian Li; Mark R Marten
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  100     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-01     Completed Date:  2008-05-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  458-65     Citation Subset:  IM    
Copyright Information:
(c) 2008 Wiley Periodicals, Inc.
Affiliation:
Department Chemical and Biochemical Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, Maryland 21250, USA.
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MeSH Terms
Descriptor/Qualifier:
Aspergillus oryzae / growth & development*,  metabolism,  ultrastructure
Carbon / metabolism*
Industrial Microbiology*
Models, Biological
Mycelium / growth & development*,  metabolism,  ultrastructure
Time Factors
Vacuoles / metabolism,  ultrastructure
Chemical
Reg. No./Substance:
7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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