| Fungal malformins inhibit bleomycin-induced G2 checkpoint in Jurkat cells. | |
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MedLine Citation:
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PMID: 17666789 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A DNA-damaging agent, bleomycin, arrests the cell cycle at the G2 phase of Jurkat cells, which are defective in the G1 checkpoint, while microtubule-disrupting colchicine arrests it at M phase. Fungal cyclopeptides, malformin A1 and malformin C, were found to abrogate bleomycin-induced G2 arrest (IC(50); 0.48 microM and 0.9 nM, respectively), resulting in a drastic decrease in cells in G2 phase and increase in cells in subG1 phase. On the other hand, malformins showed little effect on the colchicine-induced M phase arrest in Jurkat cells (IC(50); 2.7 microM and 24 nM, respectively). Malformin C (0.026 microM) also abrogated bleomycin-induced G2 arrest in colon cancer-derived HCT-116 cells. These data strongly suggest that malformin C disrupted the cell cycle at the G2 checkpoint of cancer cells, leading to sensitization of the cancer cells to the anti-cancer reagent. |
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Authors:
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Keiichi Hagimori; Takashi Fukuda; Yoko Hasegawa; Satoshi Omura; Hiroshi Tomoda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biological & pharmaceutical bulletin Volume: 30 ISSN: 0918-6158 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-08-01 Completed Date: 2007-09-21 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
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Languages: eng Pagination: 1379-83 Citation Subset: IM |
Affiliation:
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Kitasato Institute for Life Sciences & Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibiotics, Antineoplastic
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antagonists & inhibitors*,
pharmacology Aspergillus niger / drug effects, metabolism Bleomycin / antagonists & inhibitors*, pharmacology CDC2-CDC28 Kinases / metabolism Cell Cycle / drug effects Cell Line, Tumor Colchicine / pharmacology Culture Media / chemistry Fibrinolysis / drug effects G2 Phase / drug effects* Humans Immunoblotting Jurkat Cells Peptides, Cyclic / isolation & purification, pharmacology* Protein Synthesis Inhibitors / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 0/Culture Media; 0/Peptides, Cyclic; 0/Protein Synthesis Inhibitors; 11056-06-7/Bleomycin; 3022-92-2/malformins; 64-86-8/Colchicine; EC 2.7.11.22/CDC2-CDC28 Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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