Document Detail

Fungal lectin, XCL, is internalized via clathrin-dependent endocytosis and facilitates uptake of other molecules.
MedLine Citation:
PMID:  14629119     Owner:  NLM     Status:  MEDLINE    
The lectin isolated from Xerocomus chrysenteron (XCL) displays a toxic activity towards insects. In order to assess its possible mode of action and to gather useful data for its potential use in insect-resistant transgenic plants, we investigated the effects of XCL at the cellular level. Immunofluorescence microscopy studies revealed that XCL is rapidly internalized into small endocytic vesicles that further coalesce in the perinuclear region. We show that XCL is endocytosed by the clathrin-dependent pathway, and is delivered to late endosome/lysosome compartments. The internalization of XCL seems to be general since it occurs in different cell types such as insect (SF9) or mammalian (NIH-3T3 and Hela) cell lines. In the presence of XCL, the uptake of GFP and BSA is greatly enhanced, demonstrating that XCL facilitates endocytosis. Thus, XCL could serve as a delivery agent to facilitate the endocytosis of proteins that do not enter the cell alone.
Frédéric Francis; Claire Marty-Detraves; Renaud Poincloux; Laurent Baricault; Didier Fournier; Laurent Paquereau
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of cell biology     Volume:  82     ISSN:  0171-9335     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-11-21     Completed Date:  2004-07-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  515-22     Citation Subset:  IM    
Unité de Zoologie Générale et Appliquée, Faculté Universitaire des Sciences Agronomiques, Gembloux, Belgium.
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MeSH Terms
Antifungal Agents / pharmacology
Cell Line
Chlorpromazine / pharmacology
Clathrin / metabolism*
Clathrin-Coated Vesicles / metabolism
Endocytosis / physiology*
Endosomes / metabolism
Filipin / pharmacology
Fungi / drug effects,  metabolism*
Green Fluorescent Proteins
Lectins / genetics,  metabolism*
Luminescent Proteins
Lysosomes / metabolism
Potassium / metabolism
Protein Transport / physiology
Reg. No./Substance:
0/Antifungal Agents; 0/Clathrin; 0/Lectins; 0/Luminescent Proteins; 147336-22-9/Green Fluorescent Proteins; 480-49-9/Filipin; 50-53-3/Chlorpromazine; 7440-09-7/Potassium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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