Document Detail


Fundamental signals that regulate eosinophil homing to the gastrointestinal tract.
MedLine Citation:
PMID:  10377178     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The histological identification of increased eosinophils in the gastrointestinal tract occurs in numerous clinical disorders; however, there is a limited understanding of the mechanisms regulating eosinophil trafficking into this mucosal surface. The results presented in this study characterize the processes regulating eosinophil homing into the gastrointestinal tract at baseline. Eosinophils were found to be present in the lamina propria of 19-day-old embryos and germ-free adult mice at concentrations comparable to those present in non-germ-free adult mice. Furthermore, eosinophil gastrointestinal levels were not altered by increasing circulating eosinophils after pulmonary allergen challenge. Gastrointestinal eosinophil levels were partially reduced in mice deficient in recombinase activating gene-1 (RAG-1), IL-5, or the beta common chain (betac), but these reductions paralleled reductions in circulating eosinophils. In contrast, mice deficient in eotaxin had a marked reduction in gastrointestinal eosinophils but normal levels of eosinophils in the hematopoietic compartments. Furthermore, eotaxin was important for regulating eosinophil levels, even in the presence of high levels of IL-5. These investigations demonstrate eosinophil homing into the gastrointestinal tract during embryonic development occurring independently of viable intestinal flora. Furthermore, eotaxin is identified as the primary regulator of eosinophil gastrointestinal homing under homeostatic states, and may therefore have a fundamental role in innate immune responses.
Authors:
A Mishra; S P Hogan; J J Lee; P S Foster; M E Rothenberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  103     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-07-07     Completed Date:  1999-07-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1719-27     Citation Subset:  AIM; IM    
Affiliation:
Division of Pulmonary Medicine, Allergy and Clinical Immunology, Department of Pediatrics, Children's Hospital Medical Center, Cincinnati, Ohio 45229, USA.
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MeSH Terms
Descriptor/Qualifier:
Allergens / administration & dosage
Animals
Animals, Newborn / immunology
Antigens, Fungal / administration & dosage
Aspergillus fumigatus / immunology
Cell Movement / immunology*
Chemokine CCL11
Chemokines, CC*
Cytokines / biosynthesis,  physiology
Digestive System / cytology*,  immunology*,  metabolism
Eosinophilia / etiology
Eosinophils / immunology*
Granulocyte-Macrophage Colony-Stimulating Factor / physiology
Interleukin-5 / physiology
Leukopenia / etiology
Lung / immunology
Lymphopenia / etiology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred CBA
Mice, Mutant Strains
Mice, Nude
Mice, Transgenic
Grant Support
ID/Acronym/Agency:
R01 AI-42422-02/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Allergens; 0/Antigens, Fungal; 0/Ccl11 protein, mouse; 0/Chemokine CCL11; 0/Chemokines, CC; 0/Cytokines; 0/Interleukin-5; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor
Comments/Corrections

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