Document Detail

Fundamental role of ClC-3 in volume-sensitive Cl- channel function and cell volume regulation in AGS cells.
MedLine Citation:
PMID:  12842831     Owner:  NLM     Status:  MEDLINE    
Volume regulation is essential for cell function, but it is unknown which channels are involved in a regulatory volume decrease (RVD) in human gastric epithelial cells. Exposure to a hypotonic solution caused the increase in AGS cell volume, followed by the activation of a current. The reversal potential of the swelling-induced current suggested that Cl- was the primary charge carrier. The selectivity sequence for different anions was I- > Br- > Cl- > F- > gluconate. This current was inhibited by flufenamate, DIDS, tamoxifen, and 5-nitro-2-(3-phenylpropylamino)benzoate. Intracellular dialysis of three different anti-ClC-3 antibodies abolished or attenuated the Cl- current and disrupted RVD, whereas the current and RVD was unaltered by anti-ClC-2 antibody. Immunoblot studies demonstrated the presence of ClC-3 protein in Hela and AGS cells. RT-PCR analysis detected expression of ClC-3, MDR-1, and pICln mRNA in AGS cells. These results suggest a fundamental role of endogenous ClC-3 in the swelling-activated Cl- channels function and cell volume regulation in human gastric epithelial cells.
Nan Ge Jin; Jin Kyoung Kim; Dong Ki Yang; Soo Jin Cho; Jung Mogg Kim; Eun Ju Koh; Hyun Chae Jung; Insuk So; Ki Whan Kim
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-07-03
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  285     ISSN:  0193-1857     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-16     Completed Date:  2003-12-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G938-48     Citation Subset:  IM    
Dept. of Physiology and Biophysics, Seoul National Univ. College of Medicine, 28 Yongon-Dong, Chongro-Gu, Seoul, Korea 110-799.
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MeSH Terms
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
Antibodies / pharmacology
Biophysical Phenomena
Cell Line
Cell Size / physiology
Chloride Channels / antagonists & inhibitors,  genetics,  immunology,  physiology*
Chlorides / antagonists & inhibitors
Estrogen Antagonists / pharmacology
Flufenamic Acid / pharmacology
Gastric Mucosa / cytology*,  drug effects,  physiology
Hela Cells
Hypotonic Solutions / pharmacology
Ion Channels*
Nitrobenzoates / pharmacology
P-Glycoprotein / genetics
RNA, Messenger / metabolism
Tamoxifen / pharmacology
Reg. No./Substance:
0/Antibodies; 0/CLNS1A protein, human; 0/Chloride Channels; 0/Chlorides; 0/ClC-3 channel; 0/Estrogen Antagonists; 0/Hypotonic Solutions; 0/Ion Channels; 0/Nitrobenzoates; 0/P-Glycoprotein; 0/RNA, Messenger; 10540-29-1/Tamoxifen; 107254-86-4/5-nitro-2-(3-phenylpropylamino)benzoic acid; 530-78-9/Flufenamic Acid; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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