| Fundamental cryobiology of rat immature and mature oocytes: hydraulic conductivity in the presence of Me(2)SO, Me(2)SO permeability, and their activation energies. | |
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MedLine Citation:
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PMID: 10684576 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The hydraulic conductivity in the presence of dimethyl sulfoxide Me(2)SO (L(p)(Me(2)SO)), Me(2)SO (P(Me(2)SO)) permeability and reflection coefficient (sigma) of immature (germinal vesicle; GV) and mature (metaphase II; MII) rat oocytes were determined at various temperatures. A temperature controlled micropipette perfusion technique was used to conduct experiments at five different temperatures (30, 20, 10, 4, and -3 degrees C). Kedem and Katchalsky membrane transport theory was used to describe the cell volume kinetics. The cell volumetric changes of oocytes were calculated from the measurement of two oocyte diameters, assuming a spherical shape. The activation energies (E(a)) of L(p)(Me(2)SO) and P(Me(2)SO) were calculated using the Arrhenius equation. Activation energies of L(p)(Me(2)SO) for GV and MII oocytes were 34.30 Kcal/mol and 16.29 Kcal/mol, respectively; while the corresponding E(a)s of P(Me(2)SO) were 19.87 Kcal/mol and 21.85 Kcal/mol, respectively. These permeability parameters were then used to calculate cell water loss in rat oocytes during cooling at subzero temperatures. Based on these values, the predicted optimal cooling rate required to maintain extra- and intracellular water in near equilibrium for rat GV stage oocytes was found to be between 0.05 degrees C/min and 0. 025; while for rat MII oocytes, the corresponding cooling rate was 1 degrees C/min. These data suggest that standard cooling rates used for mouse oocytes (e.g., 0.5-1 degrees C/min) can also be employed to cryopreserve rat MII oocytes. However, the corresponding cooling rate required to avoid damage must be significantly slower for the GV stage rat oocyte. J. Exp. Zool. 286:523-533, 2000. |
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Authors:
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Y Agca; J Liu; E S Critser; J K Critser |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of experimental zoology Volume: 286 ISSN: 0022-104X ISO Abbreviation: J. Exp. Zool. Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-04-04 Completed Date: 2000-04-04 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0375365 Medline TA: J Exp Zool Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 523-33 Citation Subset: IM |
Copyright Information:
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Copyright 2000 Wiley-Liss, Inc. |
Affiliation:
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Cryobiology Research Institute, Wells Center for Pediatric Research, Riley Hospital for Children, Indianapolis, Indiana 46202, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Membrane Permeability / drug effects Cryopreservation / veterinary* Dimethyl Sulfoxide / pharmacology* Female Metaphase Mice Oocytes / cytology*, drug effects Osmolar Concentration Rats Rats, Sprague-Dawley Time Factors |
| Chemical | |
Reg. No./Substance:
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67-68-5/Dimethyl Sulfoxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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