| Functionally acceptable substitutions in two alpha-helical regions of lambda repressor. | |
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MedLine Citation:
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PMID: 2199970 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method of targeted random mutagenesis has been used to investigate the informational content of 25 residue positions in two alpha-helical regions of the N-terminal domain of lambda repressor. Examination of the functionally allowed sequences indicates that there is a wide range in tolerance to amino acid substitution at these positions. At positions that are buried in the structure, there are severe limitations on the number and type of residues allowed. At most surface positions, many different residues and residue types are tolerated. However, at several surface positions there is a strong preference for hydrophilic amino acids, and at one surface position proline is absolutely conserved. The results reveal the high level of degeneracy in the information that specifies a particular protein fold. |
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Authors:
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J F Reidhaar-Olson; R T Sauer |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proteins Volume: 7 ISSN: 0887-3585 ISO Abbreviation: Proteins Publication Date: 1990 |
Date Detail:
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Created Date: 1990-09-11 Completed Date: 1990-09-11 Revised Date: 2008-08-14 |
Medline Journal Info:
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Nlm Unique ID: 8700181 Medline TA: Proteins Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 306-16 Citation Subset: IM |
Affiliation:
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Department of Biology, Massachusetts Institute of Technology, Cambridge 02139. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence DNA-Binding Proteins* Escherichia coli / genetics* Membrane Proteins Molecular Sequence Data Monte Carlo Method Mutation Protein Conformation Repressor Proteins* / genetics Transcription Factors* / genetics Viral Proteins Viral Regulatory and Accessory Proteins |
| Grant Support | |
ID/Acronym/Agency:
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AI-15706/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Membrane Proteins; 0/Repressor Proteins; 0/Transcription Factors; 0/Viral Proteins; 0/Viral Regulatory and Accessory Proteins; 0/phage repressor proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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