Document Detail


Functional tissue engineering of autologous tunica albuginea: a possible graft for Peyronie's disease surgery.
MedLine Citation:
PMID:  15149752     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: The aim of the present study was to generate a tissue engineered type of mechanically stable graft suitable for surgical replacement of the tunica albuginea penis. METHODS: Porcine fibroblasts isolated from open fascia biopsies were seeded on decellularized collagen matrices and then cultivated in a bioreactor under continuous multiaxial stress for up to 21 days (n=12). Static cultures without mechanical stress served as controls. Cell proliferation, cell alignment, and de novo synthesis of extracellular matrix proteins (proteoglycans, procollagen I, elastin) in these grafts was evaluated by hematoxylin-eosin, pentachrome, and immuno-staining. Additionally, the enzymatic isolation of porcine fibroblasts from X4mm skin punch biopsies (n=8) was evaluated. RESULTS: Mechanically strained cultures of fibroblasts showed a homogeneous multilayer matrix infiltration and a regular cell alignment in the direction of strain axis after 7 days, as well as a de novo production of extracellular matrix proteins compared to the static control. A large amount of viable fibroblasts was easily obtained from small skin punch biopsies. CONCLUSION: This study shows that continuous multiaxial stimuli improve proliferation and extracellular matrix synthesis of mature fibroblasts reseeded on a biological matrix making this a feasible autologous tissue engineered graft for penile surgery. For the clinical setting fibroblasts harvested from small skin biopsies can be a comfortable cell source.
Authors:
Dirk Schultheiss; Ralf R Lorenz; Roland Meister; Mareike Westphal; Alexander I Gabouev; Heike Mertsching; Christian Biancosino; Norbert Schlote; Jörg Wefer; Michael Winkler; Christian G Stief; Udo Jonas
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European urology     Volume:  45     ISSN:  0302-2838     ISO Abbreviation:  Eur. Urol.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-05-19     Completed Date:  2004-09-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7512719     Medline TA:  Eur Urol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  781-6     Citation Subset:  IM    
Affiliation:
Department of Urology and Pediatirc Urology and Tissue Engineering Network, Hannover Medical School, Germany. schultheiss.dirk@mh-hannover.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Bioreactors
Equipment Design
Male
Penile Induration / surgery*
Penis*
Swine
Tissue Engineering* / instrumentation

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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