| Functional studies on RNA-transfected cell microarrays. | |
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MedLine Citation:
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PMID: 20387150 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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RNA-transfected cell microarray shows great promise in functional genomics. By printing siRNA complexed with transfection reagent on glass slides, arrays of transfected cells are formed in which the phenotypic consequences of gene suppression can be investigated. Using reporter plasmids with fluorescence intensity as output data, we have developed a strategy for statistical analysis of the intensity data from medium-scale functional studies using data from several experimental replicates. |
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Authors:
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Christina Saeten Fjeldbo; Kristine Misund; Clara-Cecilie G?nther; Mette Langaas; Tonje Str?mmen Steigedal; Liv Thommesen; Astrid Laegreid; Torunn Bruland |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 629 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-04-13 Completed Date: 2010-06-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 175-91 Citation Subset: IM |
Affiliation:
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Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Humans Linear Models Oligonucleotide Array Sequence Analysis / instrumentation, methods* RNA, Small Interfering / metabolism* Solutions Statistics as Topic Tissue Fixation Transfection / methods* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Small Interfering; 0/Solutions |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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