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Functional state analysis of phagocytic cells of patients with type 2 diabetes and pulmonary tuberculosis.
MedLine Citation:
PMID:  22582504     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
BACKGROUND: The phagocytic function in pulmonary tuberculosis (PTB) and Type 2 diabetes (T2D) has been explored mainly in macrophages but not in polymorphonuclears (PMN). The purpose of this study was to determine the functional status of PMN leukocytes in patients with pulmonary tuberculosis (PTB), Type 2 diabetes (T2D), and in patients with both diseases.
METHODS: An observational, prospective, and comparative study was carried out. 30 ambulatory patients with T2D, 10 with PTB undergoing treatment and 10 patients with PTB and T2D, and 44 healthy subjects were studied. PMN leukocytes were separated, the capacity of these cells to produce hydrogen peroxide and to reduce nitroblue tetrazolium (NBT) in response to stimulus with the phorbolic ester of myristic acid (PMA) was measured; and the capacity of PMN leukocytes to adhere to surfaces was determined.
RESULTS: Concerning the test for adherence, on comparing healthy subjects with patients with T2D+PTB, we observed a clear decrease in cellular adherence in the group of patients with both diseases; it was statistically significant (p = 0.007).With regard to phagocytic function, we observed that in NBT reduction as well as in hydrogen peroxide production, statistically significant differences were not obtained on comparing healthy subjects with any of the three groups of patients.
CONCLUSIONS: We observed a clear decrease in cellular adherence when both diseases co-exist. These results could indicate the need for the co-existence of T2D and TB to cause deterioration in the cells' adherence activity. The microtechniques employed permit the evaluation in a practical manner of certain phagocytic-activity expressions.
Authors:
Melby Mendoza-Aguilar; Guadalupe García-Elorriaga; Patricia Arce-Paredes; César González-Bonilla; Guillermo Del Rey-Pineda; Oscar Rojas-Espinosa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical laboratory     Volume:  58     ISSN:  1433-6510     ISO Abbreviation:  Clin. Lab.     Publication Date:  2012  
Date Detail:
Created Date:  2012-05-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9705611     Medline TA:  Clin Lab     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  299-305     Citation Subset:  IM    
Affiliation:
Unidad de Investigación Mdica en Inmunología e Infectología, Hospital de Infectología, Centro Médico Nacional La Raza (CMNR), Instituto Mexicano del Seguro Social (IMSS), Mexico City, Mexico.
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