Document Detail


Functional significance of the kainate receptor GluR6(M836I) mutation that is linked to autism.
MedLine Citation:
PMID:  17167233     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies revealed a linkage of the kainate receptor GluR6 with autism, a pervasive developmental disorder. Mutational screening in autistic patients disclosed the amino acid exchange M836I in a highly conserved domain of the cytoplasmic C-terminal region of GluR6. Here, we show that this mutation leads to GluR6 gain-of-function. By using the two-electrode voltage clamp technique we observed a significant increase of current amplitudes of mutant GluR6 compared to wild type GluR6. Western blotting of oocytes injected with mutant or wild type GluR6 cRNA and transfection of EGFP-tagged GluR6 receptors into COS-7 cells revealed an enhanced plasma membrane expression of GluR6(M836I) compared to wild type GluR6. Membrane expression of GluR6(M836I) but not of wild type GluR6 seems to be regulated by Rab11 as indicated by our finding that GluR6(M836I) but not wild type GluR6 showed increased current amplitudes and protein expression when coexpressed with Rab11. Furthermore, injection of GTP plus Rab11A protein into oocytes increased current amplitudes in GluR6(M836I) but not in wild type GluR6. By contrast, Rab5 downregulated the currents in oocytes expressing wild type GluR6 but had only little, statistically not significant effects on currents in oocytes expressing GluR6(M836I). Our data on altered functional properties of GluR6(M836I) provide a functional basis for the postulated linkage of GluR6 to autism. Furthermore, we identified new mechanisms determining the plasma membrane abundance of wild type GluR6 and GluR6(M836I).
Authors:
Nathalie Strutz-Seebohm; Ganna Korniychuk; Regina Schwarz; Ravshan Baltaev; Oana N Ureche; Andreas F Mack; Zhan-Lu Ma; Michael Hollmann; Florian Lang; Guiscard Seebohm
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  18     ISSN:  1015-8987     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2006  
Date Detail:
Created Date:  2006-12-14     Completed Date:  2007-02-13     Revised Date:  2010-01-13    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  287-94     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2006 S. Karger AG, Basel.
Affiliation:
Department of Physiology I, University of Tuebingen, Tuebingen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Substitution
Animals
Autistic Disorder / genetics*,  metabolism
COS Cells
Cell Membrane / chemistry,  metabolism*
Cercopithecus aethiops
Humans
Mutation
Oocytes
Patch-Clamp Techniques
Receptors, Kainic Acid / analysis,  genetics,  metabolism*
Transfection
Xenopus laevis
rab GTP-Binding Proteins / metabolism*
rab5 GTP-Binding Proteins / metabolism*
Chemical
Reg. No./Substance:
0/Gluk2 kainate receptor; 0/Receptors, Kainic Acid; EC 3.6.1.-/rab GTP-Binding Proteins; EC 3.6.1.-/rab11 protein; EC 3.6.5.2/rab5 GTP-Binding Proteins

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