Document Detail

Functional roles of the hook in a rotating tethered cell.
MedLine Citation:
PMID:  18031758     Owner:  NLM     Status:  MEDLINE    
A bacterial cell tethered through a flagellum on a glass slide rotates its cell body in either counter-clockwise or clockwise at around 10 Hz. To analyze the detailed manner of rotation, we have constructed and expressed yellow fluorescent protein (YFP)-FliN fusion protein in a fliN deletion mutant, resulting in the recovery of motility of the Fla(-) mutant cells. The tethered cells that incorporated the fusion protein in the flagellar motor rotate around one of the fluorescent spots. Tracing the center spot of a rotating cell, we have found that the rotating circles of the tethered cells were often distorted, and that the cell has seldom rotated smoothly but gyrated around the center point. The radii of the gyrating circles were 100-200 nm for the wild-type cells, and 50 nm for the cells carrying short hooks, suggesting that the flexibility of the hook is responsible for asymmetrical rotation. These observations indicate that tethered cells almost always interact with the glass surface in one cycle of rotation, where the length and flexibility of the hook have an important role.
Manami Hashimoto; Takuji Mashimo; Takanori Hirano; Shigeru Yamaguchi; Shin-ichi Aizawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-09-20
Journal Detail:
Title:  Journal of molecular biology     Volume:  375     ISSN:  1089-8638     ISO Abbreviation:  J. Mol. Biol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-10     Completed Date:  2007-12-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985088R     Medline TA:  J Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  367-75     Citation Subset:  IM    
CREST, Japan Science and Technology Agency, c/o Innovation Plaza Hiroshima, 3-10-23 Kagamiyama, Higashi-Hiroshima, 739-0046, Japan.
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MeSH Terms
Bacterial Proteins / genetics,  metabolism,  physiology
Cell Movement
Escherichia coli / genetics,  physiology*
Flagella / physiology,  ultrastructure
Gene Deletion
Glass / chemistry
Isopropyl Thiogalactoside / pharmacology
Luminescent Proteins / metabolism
Microscopy, Fluorescence
Models, Biological
Particle Size
Recombinant Fusion Proteins / metabolism
Salmonella typhimurium / genetics,  physiology*
Time Factors
Reg. No./Substance:
0/Bacterial Proteins; 0/FliN protein, Bacteria; 0/Luminescent Proteins; 0/Recombinant Fusion Proteins; 0/yellow fluorescent protein, Bacteria; 367-93-1/Isopropyl Thiogalactoside

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