Document Detail


Functional role of beta 1 integrin-mediated signalling in the human hair follicle.
MedLine Citation:
PMID:  18155697     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Integrins are transmembrane adhesion proteins that convey critical topobiological information and exert crucial signalling functions. In skin and hair follicle biology, beta1 integrins and their ligands are of particular interest. It is not yet known whether beta1 integrins play any role in the regulation of human hair growth and the expression pattern of beta1 integrin in the human pilosebaceous unit remains ill-defined. Here, we show that pilosebaceous immunoreactivity for beta1 integrin is most prominent in the outermost layer of the outer root sheath and the surrounding connective tissue sheath of human scalp hair follicles in situ and in vitro. Sites of beta1 integrin immunoreactivity co-express fibronectin and tenascin-C. Contrary to previous reports, beta1 integrin immunoreactivity in situ was not significantly upregulated in the human bulge region. Functionally, two beta1 integrin-activating antibodies (12G10, TS2/16) and ligand-mimicking RGD peptides promoted the growth of microdissected, organ-cultured human scalp hair follicles in vitro and inhibited spontaneous hair follicle regression. This supports the concept that beta1 integrin-mediated signalling is also important in human hair growth control. The physiologically relevant organ culture assay employed here is a potential research tool for exploring whether targeted stimulation of beta1 integrin-mediated signalling is a suitable candidate for human hair loss management.
Authors:
J E Kloepper; S Hendrix; E Bodó; S Tiede; M J Humphries; M P Philpott; R Fässler; R Paus
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-12
Journal Detail:
Title:  Experimental cell research     Volume:  314     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-24     Completed Date:  2008-04-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  498-508     Citation Subset:  IM    
Affiliation:
Department of Dermatology, Allergology and Venerology, University Hospital of Schleswig-Holstein, University of Lübeck, Lübeck, Germany. Jennifer.Kloepper@uk-sh.de <Jennifer.Kloepper@uk-sh.de>
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MeSH Terms
Descriptor/Qualifier:
Antibodies / pharmacology
Antigens, CD29 / drug effects,  genetics,  metabolism*
Biological Assay / methods
Cells, Cultured
Connective Tissue / metabolism,  ultrastructure
Epithelial Cells / drug effects,  metabolism,  ultrastructure
Female
Fibronectins / metabolism
Hair / drug effects,  growth & development,  metabolism
Hair Follicle / drug effects,  metabolism*,  ultrastructure
Humans
Immunohistochemistry
Ligands
Organ Culture Techniques
Peptides / pharmacology
Signal Transduction / physiology*
Tenascin / metabolism
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens, CD29; 0/Fibronectins; 0/Ligands; 0/Peptides; 0/Tenascin

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