| Functional retinoid receptors in budding ascidians. | |
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MedLine Citation:
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PMID: 10831038 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A homolog of retinoid X receptors (RXR), named PmRXR, was cloned from the budding ascidian, Polyandrocarpa misakiensis. Gel-shift assays revealed that PmRXR and a previously identified P. misakiensis retinoic acid receptor (PmRAR) formed a complex to bind vertebrate-type retinoic acid response element (RARE). Transfection assays were carried out using a reporter gene containing a RARE upstream of lacZ. Two chimeric effector genes were constructed by placing PmRXR and PmRAR cDNA fragments (containing the DNA-binding, ligand-binding and ligand-dependent transactivation domains) downstream of the human RXR alpha and RAR alpha cDNA (covering the N-terminal coding region), respectively. Each chimeric cDNA was ligated to a notochord-specific enhancer. In case the embryos were transfected with all three transgenes and treated with retinoic acid (RA), the reporter gene was activated in the notochord cells. The result suggests that the PmRXR/PmRAR complex functions as an RA-dependent transcriptional activator. The PmRXR mRNA was detected in a mesenchymal cell type, called glomerulocyte, in the developing Polyandrocarpa bud. As this cell type has been shown to express PmRAR mRNA, it seems possible that the PmRXR/PmRAR complex mediates RA signaling in this cell type to induce the expression of genes involved in the morphogenesis of the developing bud. |
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Authors:
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M Kamimura; S Fujiwara; K Kawamura; T Yubisui |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Development, growth & differentiation Volume: 42 ISSN: 0012-1592 ISO Abbreviation: Dev. Growth Differ. Publication Date: 2000 Feb |
Date Detail:
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Created Date: 2000-08-22 Completed Date: 2000-08-22 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0356504 Medline TA: Dev Growth Differ Country: JAPAN |
Other Details:
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Languages: eng Pagination: 1-8 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular and Cellular Biotechnology Faculty of Science, Kochi University, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals DNA / metabolism Humans Molecular Sequence Data RNA, Messenger / metabolism Receptors, Retinoic Acid / classification, genetics*, metabolism, physiology Retinoid X Receptors Sequence Homology, Amino Acid Transcription Factors / classification, genetics*, metabolism, physiology Transcriptional Activation Urochordata / embryology, genetics* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Receptors, Retinoic Acid; 0/Retinoid X Receptors; 0/Transcription Factors; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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