Document Detail

Functional relationships between Rad18 and WRNIP1 in vertebrate cells.
MedLine Citation:
PMID:  17077513     Owner:  NLM     Status:  MEDLINE    
The WRNIP1 protein interacts with WRN, the product of the causative gene for Werner syndrome. Mutation of the Saccharomyces cerevisiae gene MGS1, the yeast counterpart of WRNIP1, confers synthetic lethality with mutation of RAD18. To examine the functional relationship between WRNIP1 and Rad18 in higher eukaryotic cells, we generated WRNIP1-/-/-/RAD18-/- lines from chicken DT40 cells and compared them with single mutant cell lines. Unlike the corresponding yeast mutant, WRNIP1-/-/-/RAD18-/- cells are viable but grow more slowly than single mutants and wild type cells, and they show an additive or synergistic elevation in the frequency of sister chromatid exchanges. As reported, WRNIP1-/-/- cells and RAD18-/- cells are moderately and severely sensitive to camptothecin (CPT), respectively. Unexpectedly, the severe CPT sensitivity of RAD18-/- cells is slightly suppressed by disruption of the WRNIP1 gene.
Akari Yoshimura; Masayuki Seki; Tomoko Hayashi; Yumiko Kusa; Shusuke Tada; Yutaka Ishii; Takemi Enomoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  29     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-01     Completed Date:  2007-02-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2192-6     Citation Subset:  IM    
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
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MeSH Terms
Antineoplastic Agents, Phytogenic / pharmacology
B-Lymphocytes / cytology,  drug effects,  metabolism
Camptothecin / pharmacology
Cell Line
Cell Survival / drug effects,  genetics,  physiology
Cisplatin / pharmacology
DNA Replication / drug effects
DNA-Binding Proteins / genetics,  physiology*
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Methyl Methanesulfonate / pharmacology
Mutation / genetics*
Recombination, Genetic / drug effects
Sister Chromatid Exchange / drug effects
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/DNA-Binding Proteins; 0/Enzyme Inhibitors; 0/RAD18 protein, human; 15663-27-1/Cisplatin; 66-27-3/Methyl Methanesulfonate; 7689-03-4/Camptothecin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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