| Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment. | |
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MedLine Citation:
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PMID: 19211913 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is important to maintain internal pH homeostasis in biological systems. In our previous studies, H(+)-ATPase-rich (HR) cells were found to be responsible for proton secretion in the skin of zebrafish embryos during development. In this study, zebrafish embryos were exposed to acidic and basic waters to investigate the regulation of HR cell acid secretion during pH disturbances. Our results showed that the function of HR cells on the skin of zebrafish embryos can be upregulated in pH 4 water not only by increasing the cell number but also by enlarging the acid-secreting function of single cells. We also identified an "alveolar-type" apical opening under scanning electron microscopy observations of the apical membrane of HR cells, and the density and size of the alveolar type of apical openings were also increased in pH 4 water. p63 and PCNA immunostaining results also showed that additional HR cells in pH 4 water may be differentiated not only from ionocyte precursor cells but also newly proliferating epithelial stem cells. |
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Authors:
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Jiun-Lin Horng; Li-Yih Lin; Pung-Pung Hwang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-11 |
Journal Detail:
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Title: American journal of physiology. Cell physiology Volume: 296 ISSN: 0363-6143 ISO Abbreviation: Am. J. Physiol., Cell Physiol. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-01 Completed Date: 2009-06-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901225 Medline TA: Am J Physiol Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: C682-92 Citation Subset: IM |
Affiliation:
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Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan, Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acid-Base Equilibrium* Adaptation, Physiological Animals Apoptosis Cell Differentiation Cell Proliferation Embryonic Stem Cells / enzymology*, ultrastructure Epithelial Cells / enzymology*, ultrastructure Fresh Water / chemistry Hydrogen-Ion Concentration Immunohistochemistry Microscopy, Confocal Microscopy, Electron, Scanning Phosphoproteins / metabolism Proliferating Cell Nuclear Antigen / metabolism Proton-Translocating ATPases / metabolism* Skin / embryology, enzymology*, ultrastructure Time Factors Trans-Activators / metabolism Zebrafish / embryology, metabolism* Zebrafish Proteins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Phosphoproteins; 0/Proliferating Cell Nuclear Antigen; 0/Trans-Activators; 0/Zebrafish Proteins; 0/tp73l protein, zebrafish; EC 3.6.3.14/Proton-Translocating ATPases |
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