| Functional proteomic analysis of lipases and esterases in cultured human adipocytes. | |
| | |
MedLine Citation:
|
PMID: 20942458 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
This study reports on the analysis of the lipolytic proteome of cultured human fat cells. We used specific affinity tags to detect and identify the lipolytic and esterolytic enzymes in human subcutaneous (Sc) and visceral (Visc) adipocytes. For this purpose, differentiated fat cells were incubated with a fluorescent suicide inhibitor followed by protein separation using one- or two-dimensional gel electrophoresis. After detection by fluorescence laser scanning, the labeled proteins were tryptically digested and peptides were identified by mass spectrometry. In addition, a biotinylated probe was used for specific enzyme labeling with subsequent avidin affinity isolation of the tagged proteins. Finally, we determined the quantitative differences in protein expression levels between subcutaneous and visceral adipocytes using differential activity-based gel electrophoresis (DABGE). We found that the lipase/esterase patterns of both cell types are very similar, except for some proteins that were only found in Sc cells. Two novel enzyme candidates identified in this study were overexpressed and characterized using biologically relevant glycerolipid substrates in vitro. Both of them showed pronounced hydrolytic activities on hydrophobic acylglycerols and therefore may be considered lipases. The physiological functions of the novel lipolytic proteins in vivo are currently subject to investigation. |
| | |
Authors:
|
Maximilian Schicher; Maria Morak; Ruth Birner-Gruenberger; Heidemarie Kayer; Bojana Stojcic; Gerald Rechberger; Manfred Kollroser; Albin Hermetter |
Related Documents
:
|
16880928 - A novel peptide microarray for protein detection and analysis utilizing a dry peptide a... 15979338 - Multidimensional protein identification technology (mudpit): technical overview of a pr... 18021238 - Applications of diagonal chromatography for proteome-wide characterization of protein m... 19764808 - Modified spectral count index (msci) for estimation of protein abundance by protein rel... 12234008 - Gluconacetobacter diazotrophicus, a sugar cane endosymbiont, produces a bacteriocin aga... 16880928 - A novel peptide microarray for protein detection and analysis utilizing a dry peptide a... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-10 |
Journal Detail:
|
Title: Journal of proteome research Volume: 9 ISSN: 1535-3907 ISO Abbreviation: J. Proteome Res. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-12-03 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: United States |
Other Details:
|
Languages: eng Pagination: 6334-44 Citation Subset: IM |
Affiliation:
|
Institute of Biochemistry, Graz University of Technology, Graz, Austria. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Development of EGFR-Targeted Polymer Blend Nanocarriers for Combination Paclitaxel/Lonidamine Delive...
Next Document: Yield, Content, and Composition of Peppermint and Spearmints as a Function of Harvesting Time and Dr...