Document Detail


Functional properties of human neuronal Kv11 channels.
MedLine Citation:
PMID:  19319565     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Kv11 potassium channels are important for regulation of the membrane potential. Kv11.2 and Kv11.3 are primarily found in the nervous system, where they most likely are involved in the regulation of neuronal excitability. Two isoforms of human Kv11.2 have been published so far. Here, we present a new splice variant that is present in human brain as demonstrated by reverse transcription PCR. Heterologous expression in Xenopus laevis oocytes revealed a 30-mV shift in the voltage dependence of activation to more depolarized potentials and slower activation together with faster deactivation kinetics compared to hKv11.1. Further, we have cloned and electrophysiologically characterized two splice variants of hKv11.3. When expressed in X. laevis oocytes, both isoform 1 and isoform 2 elicited robust currents with a striking transient current component caused by delayed inactivation. The different current characteristics of the isoforms presented in this work may contribute to the regulation of neuronal excitability.
Authors:
Karoline Einarsen; Kirstine Calloe; Morten Grunnet; Søren-Peter Olesen; Nicole Schmitt
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-25
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  458     ISSN:  1432-2013     ISO Abbreviation:  Pflugers Arch.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-01     Completed Date:  2009-09-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  689-700     Citation Subset:  IM    
Affiliation:
The Danish National Research Foundation Centre for Cardiac Arrhythmia, Department of Biomedical Sciences, The Panum Institute, University of Copenhagen, Blegdamsvej 3, 2200, Copenhagen N, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Action Potentials / physiology*
Animals
Cells, Cultured
Ion Channel Gating / physiology*
Membrane Potentials / physiology*
Neurons / physiology*
Oocytes / physiology*
Potassium Channels / chemistry,  genetics,  physiology*
Xenopus laevis
Chemical
Reg. No./Substance:
0/Potassium Channels

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