| Functional nucleic acids in high throughput screening and drug discovery. | |
| | |
MedLine Citation:
|
PMID: 18045081 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In vitro selection can be used to generate functional nucleic acids such as aptamers and ribozymes that can recognize a variety of molecules with high affinity and specificity. Most often these recognition events are associated with structural alterations that can be converted into detectable signals. Several signaling aptamers and ribozymes constructed by both design and selection have been successfully utilized as sensitive detection reagents. Here we summarize the development of different types of signaling nucleic acids, and approaches that have been implemented in the screening format. |
| | |
Authors:
|
Seergazhi G Srivatsan; Michael Famulok |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
|
Title: Combinatorial chemistry & high throughput screening Volume: 10 ISSN: 1386-2073 ISO Abbreviation: Comb. Chem. High Throughput Screen. Publication Date: 2007 Sep |
Date Detail:
|
Created Date: 2007-11-29 Completed Date: 2008-03-28 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9810948 Medline TA: Comb Chem High Throughput Screen Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 698-705 Citation Subset: IM |
Affiliation:
|
LIMES Institute, Program Unit Chemical Biology and Medicinal Chemistry, c/o Kekulé-Institut für Organische Chemie und Biochemie, Gerhard Domagk-Str. 1, D-53121 Bonn, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Allosteric Regulation
/
genetics,
physiology Aptamers, Nucleotide / chemistry, genetics, metabolism Biosensing Techniques* Combinatorial Chemistry Techniques* Drug Design* Models, Molecular Nucleic Acids / chemistry*, genetics, metabolism* RNA, Catalytic / chemistry, genetics, metabolism Ribosomes / chemistry, genetics, metabolism Signal Transduction / genetics, physiology |
| Chemical | |
Reg. No./Substance:
|
0/Aptamers, Nucleotide; 0/Nucleic Acids; 0/RNA, Catalytic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Functional genomics and NMR spectroscopy.
Next Document: Applications of protein microarray technology.