Document Detail


Functional molecular morphology of anterior pituitary cells, from hormone production to intracellular transport and secretion.
MedLine Citation:
PMID:  21717308     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Combined in situ hybridization (ISH) and immunohistochemistry (IHC) under electron microscopy (EM-ISH & IHC) has sufficient ultrastructural resolution to provide two-dimensional images of subcellular localization of pituitary hormone and its mRNA in a pituitary cell. The advantages of semiconductor nanocrystals (Quantum dots; Qdots) and confocal laser scanning microscopy (CLSM) enable us to obtain three-dimensional images of the subcellular localization of pituitary hormone and its mRNA. Both EM-ISH & IHC and ISH & IHC using Qdots and CLSM are useful for understanding the relationship between protein and mRNA simultaneously in two or three dimensions. CLSM observation of rab3B and SNARE proteins such as SNAP-25 and syntaxin revealed that both rab3B and SNARE system proteins play an important role and work together as the exocytotic machinery in anterior pituitary cells. Another important issue is the intracellular transport and secretion of pituitary hormone. An experimental pituitary cell line, the GH3 cell, in which growth hormone (GH) is linked to enhanced yellow fluorescein protein (EYFP), has been developed. This stable GH3 cell secretes GH linked to EYFP upon being stimulated by Ca(2+) influx or Ca(2+) release from storage. This GH3 cell is useful for real-time visualization of the intracellular transport and secretion of GH. These three methods enable us to visualize consecutively the processes of transcription, translation, transport, and secretion of pituitary hormone.
Authors:
Akira Matsuno; Akiko Mizutani; Hiroko Okinaga; Koji Takano; So Yamada; Shoko M Yamada; Hiroshi Nakaguchi; Katsumi Hoya; Mineko Murakami; Masato Takeuchi; Mutsumi Sugaya; Johbu Itoh; Susumu Takekoshi; R Yoshiyuki Osamura
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Publication Detail:
Type:  Journal Article     Date:  2011-06-30
Journal Detail:
Title:  Medical molecular morphology     Volume:  44     ISSN:  1860-1499     ISO Abbreviation:  Med Mol Morphol     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101239023     Medline TA:  Med Mol Morphol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  63-70     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Teikyo University Chiba Medical Center, 3426-3 Anesaki, Ichihara, Chiba, 299-0111, Japan, akirakun@med.teikyo-u.ac.jp.
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