Document Detail

Functional and molecular adaptation of Cl/HCO3- exchanger to chronic alkaline media in renal cells.
MedLine Citation:
PMID:  16301827     Owner:  NLM     Status:  MEDLINE    
The Cl(-)/HCO3- exchanger (AE) is one of the mechanisms that cells have developed to adjust pH Despite its importance, the role of AE isoforms in controlling steady-state pH during alkalosis has not been widely investigated. In the present study, we have evaluated whether conditions simulating acute and chronic metabolic alkalosis affected the transport activity and protein levels of Cl-/HCO3- exchangers in a rat cortical collecting duct cell line (RCCD1). pH(i) was monitored using the fluorescent dye BCECF in monolayers grown on permeable supports. Anion exchanger function was assessed by the response of pH(i) to acute chloride removal. RT-PCR and immunoblot assays were also performed. Our results showed that RCCD1 cells express two members of the anion exchanger gene family: AE2 and AE4. Functional studies demonstrated that while in acute alkalosis pH(i) became alkaline and was not regulated, after 48 h adaptation; steady-state pH(i) reached a value similar to the physiological one. Chronic treated cells also resulted in a 3-fold rise in Cl(-)/HCO3- exchange activity together with a 2.2-fold increase in AE2, but not AE4, protein abundance. We conclude that RCCD1 cells can adapt to chronic extracellular alkalosis reestablishing its steady-state pH(i) and that AE2 would play a key role in cell homeostasis.
Valeria Rivarola; Paula Ford; Osvaldo Chara; Mario Parisi; Claudia Capurro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  16     ISSN:  1015-8987     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2005  
Date Detail:
Created Date:  2005-11-22     Completed Date:  2006-01-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  271-80     Citation Subset:  IM    
Copyright Information:
(c) 2005 S. Karger AG, Basel
Laboratorio de Biomembranas, Departamento de Fisiologia y Biofisica, Facultad de Medicina, Universidadde Buenos Aires, Argentina.
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MeSH Terms
Adaptation, Physiological
Cell Line
Chloride-Bicarbonate Antiporters / metabolism*
Culture Media
Hydrogen-Ion Concentration
Kidney Tubules, Collecting / drug effects,  metabolism*
Sodium Bicarbonate / pharmacology
Reg. No./Substance:
0/Chloride-Bicarbonate Antiporters; 0/Culture Media; 144-55-8/Sodium Bicarbonate

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