| Functional mapping of an AcNPV immediately early gene which augments expression of the IE-1 trans-activated 39K gene. | |
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MedLine Citation:
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PMID: 3277328 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An early gene which augments the expression of the delayed early/late 39K gene of the baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) was identified by functional mapping. Transient expression of the plasmid p39CAT, containing the bacterial chloramphenicol acetyltransferase coding sequences under the control of the promoter of the 39K protein, was observed in cells cotransfected with AcNPV DNA digested with several restriction endonucleases. However, when p39CAT was cotransfected with viral DNA digested with Bg/II restriction endonuclease, no CAT activity could be detected. To map the location of the Bg/II-sensitive sequences required for efficient expression of 39CAT, p39CAT and Bg/II-digested viral DNA were cotransfected with a PstI library of AcNPV DNA. The PstI-N fragment restored 39CAT activity. A major early 1.3-kb transcript from this fragment was mapped by S1 nuclease analysis. Transient assay experiments indicated that this major transcript of the PstI-N fragment was produced by an immediate early gene, named IE-N. The PstI-N fragment alone did not activate expression of p39CAT but was required when IE-1 was present in limiting quantities. |
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Authors:
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D D Carson; L A Guarino; M D Summers |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Virology Volume: 162 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 1988 Feb |
Date Detail:
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Created Date: 1988-03-11 Completed Date: 1988-03-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 444-51 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Biophysics, Texas A&M University, College Station. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromosome Mapping Gene Expression Regulation* Genes, Viral* Insect Viruses / genetics* Promoter Regions, Genetic RNA, Messenger / genetics Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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