Document Detail


Functional interplay between the cell cycle and cell phenotypes.
MedLine Citation:
PMID:  23319145     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell cycle distribution of adherent cells is typically assessed using flow cytometry, which precludes the measurements of many cell properties and their cycle phase in the same environment. Here we develop and validate a microscopy system to quantitatively analyze the cell-cycle phase of thousands of adherent cells and their associated cell properties simultaneously. This assay demonstrates that population-averaged cell phenotypes can be written as a linear combination of cell-cycle fractions and phase-dependent phenotypes. By perturbing the cell cycle through inhibition of cell-cycle regulators or changing nuclear morphology by depletion of structural proteins, our results reveal that cell cycle regulators and structural proteins can significantly interfere with each other's prima facie functions. This study introduces a high-throughput method to simultaneously measure the cell cycle and phenotypes at single-cell resolution, which reveals a complex functional interplay between the cell cycle and cell phenotypes.
Authors:
Wei-Chiang Chen; Pei-Hsun Wu; Jude M Phillip; Shyam B Khatau; Jae Min Choi; Matthew R Dallas; Konstantinos Konstantopoulos; Sean X Sun; Jerry S H Lee; Didier Hodzic; Denis Wirtz
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Integrative biology : quantitative biosciences from nano to macro     Volume:  5     ISSN:  1757-9708     ISO Abbreviation:  Integr Biol (Camb)     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-25     Completed Date:  2013-09-24     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  101478378     Medline TA:  Integr Biol (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  523-34     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Cell Adhesion
Cell Culture Techniques / methods*
Cell Cycle*
Cell Line
Cell Line, Tumor
Cell Nucleus / metabolism
Cyclin-Dependent Kinase 4 / metabolism
Cytoplasm / metabolism
DNA / metabolism
Flow Cytometry
Humans
Lamin Type A / metabolism
Mice
Microscopy, Fluorescence / methods*
Models, Biological
Phenotype
Grant Support
ID/Acronym/Agency:
R01 CA174388/CA/NCI NIH HHS; R01 EY022632/EY/NEI NIH HHS; R01 GM075305/GM/NIGMS NIH HHS; R01 GM084204/GM/NIGMS NIH HHS; R25 CA153952/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Lamin Type A; 9007-49-2/DNA; EC 2.7.11.22/CDK4 protein, human; EC 2.7.11.22/Cyclin-Dependent Kinase 4
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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