Document Detail


Functional interaction between KChIP1 and GFP-fused Kv4.3L co-expressed in HEK293 cells.
MedLine Citation:
PMID:  11976919     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The trafficking and electrophysiological characteristics of Kv4 subfamily are regulated by K+-channel-interacting proteins (KChIPs), which bind to the N-terminus of Kv4. We examined in HEK293 expression system whether the fusion of a green fluorescence protein (GFP) with Kv4.3L at the N-terminus would affect the functional interaction of KChIP1 with Kv4.3L. GFP-fused Kv4.3L showed A-type K+ current (I(A)) with significantly slower recovery from inactivation (tau=218 and 496 ms) and much lower density than those of original Kv4.3L expressed in HEK293 cells. The co-expression of KChIP1 with Kv4.3L strikingly increased the density of I(A) and hastened the recovery from inactivation (tau=133 ms). Surprisingly, co-expression of KChIP1 with GFP-fused Kv4.3L markedly enhanced the current density and hastened the recovery (tau=135 ms), just as the co-expression of KChIP1 with Kv4.3L did. In conclusion, the fusion of GFP to the N-terminus of Kv4.3L per se changed the channel kinetics but did not affect the functional interaction of KChIP1 with Kv4.3L at all. The trafficking of Kv4.3L by KChIP1 to the cell membrane was visualized with GFP fusion to the N-terminus without any significant modification of changes in channel kinetics and density.
Authors:
Noriyuki Hatano; Susumu Ohya; Yuji Imaizumi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-02-27
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  444     ISSN:  0031-6768     ISO Abbreviation:  Pflugers Arch.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-04-26     Completed Date:  2002-11-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  80-8     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabedori, Mizuhoku, 467-8603, Japan.
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MeSH Terms
Descriptor/Qualifier:
Calcium Signaling / physiology
Calcium-Binding Proteins / genetics*,  metabolism*
Cell Line
DNA, Complementary / genetics
Electrophysiology
Green Fluorescent Proteins
Humans
Image Processing, Computer-Assisted
Immunohistochemistry
Indicators and Reagents
Kv Channel-Interacting Proteins
Luminescent Proteins / diagnostic use*
Microscopy, Confocal
Patch-Clamp Techniques
Potassium Channels / genetics*,  metabolism*
Potassium Channels, Voltage-Gated*
Shal Potassium Channels
Transfection
Chemical
Reg. No./Substance:
0/Calcium-Binding Proteins; 0/DNA, Complementary; 0/Indicators and Reagents; 0/KCND3 protein, human; 0/KCNIP1 protein, human; 0/Kv Channel-Interacting Proteins; 0/Luminescent Proteins; 0/Potassium Channels; 0/Potassium Channels, Voltage-Gated; 0/Shal Potassium Channels; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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