Document Detail


Functional inhibition in direction-selective retinal ganglion cells: spatiotemporal extent and intralaminar interactions.
MedLine Citation:
PMID:  12163551     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We recorded from ON-OFF direction-selective ganglion cells (DS cells) in the rabbit retina to investigate in detail the inhibition that contributes to direction selectivity in these cells. Using paired stimuli moving sequentially across the cells' receptive fields in the preferred direction, we directly confirmed the prediction of that a wave of inhibition accompanies any moving excitatory stimulus on its null side, at a fixed spatial offset. Varying the interstimulus distance, stimulus size, luminance, and speed yielded a spatiotemporal map of the strength of inhibition within this region. This "null" inhibition was maximal at an intermediate distance behind a moving stimulus: 1/2 to 11/2 times the width of the receptive field. The strength of inhibition depended more on the distance behind the stimulus than on stimulus speed, and the inhibition often lasted 1-2 s. These spatial and temporal parameters appear to account for the known spatial frequency and velocity tuning of ON-OFF DS cells to drifting contrast gratings. Stimuli that elicit distinct ON and OFF responses to leading and trailing edges revealed that an excitatory response of either polarity could inhibit a subsequent response of either polarity. For example, an OFF response inhibited either an ON or OFF response of a subsequent stimulus. This inhibition apparently is conferred by a neural element or network spanning the ON and OFF sublayers of the inner plexiform layer, such as a multistratified amacrine cell. Trials using a stationary flashing spot as a probe demonstrated that the total amount of inhibition conferred on the DS cell was equivalent for stimuli moving in either the null or preferred direction. Apparently the cell does not act as a classic "integrate and fire" neuron, summing all inputs at the soma. Rather, computation of stimulus direction likely involves interactions between excitatory and inhibitory inputs in local regions of the dendrites.
Authors:
Steven F Stasheff; Richard H Masland
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurophysiology     Volume:  88     ISSN:  0022-3077     ISO Abbreviation:  J. Neurophysiol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-06     Completed Date:  2002-09-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375404     Medline TA:  J Neurophysiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1026-39     Citation Subset:  IM    
Affiliation:
Department of Neurology, Children's Hospital, Harvard Medical School, Boston 02115, USA. sfs@massmed.org
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MeSH Terms
Descriptor/Qualifier:
Action Potentials
Animals
Electrophysiology
Female
Fluorescent Dyes
Isoquinolines
Male
Neural Inhibition / physiology*
Rabbits
Retinal Ganglion Cells / physiology*
Visual Perception / physiology
Grant Support
ID/Acronym/Agency:
NS-01701-09/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Isoquinolines; 77944-88-8/lucifer yellow

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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