| Functional and immunological analysis of the human sperm proteome. | |
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MedLine Citation:
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PMID: 22459723 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This is a review of ten previously published studies of the human sperm proteome. Proteins expressed on the sperm cell surface were identified and characterized by a combination of vectorial labelling with radioiodine and biotin, PI-PLC treatment, two-dimensional gel electrophoresis, immuno and lectin blotting procedures, affinity overlay assays with radioactive nucleotide triphosphates and 45Ca, and mass spectrometry analysis. Examination of capacitation-induced modifications of the human sperm proteome led to the cloning and characterisation of two new phospho-regulated cancer-testis antigens, which we named Fibrous Sheath Protein 95 (FSP95) and CABYR (calcium-binding tyrosine phosphorylation regulated). A protein kinase A RII binding domain is present between amino acids 124 and 141 identifying FSP95 (now commonly known as AKAP3) as a member of the A kinase anchoring protein-family which provides spatial and temporal specificity to the cAMP-PKA pathway. In addition to scaffolding PKA, PDE and protein phosphatases, AKAPs also bind to a group of four proteins that share homology to the RII dimerization/docking (R2D2) domain of PKA' regulatory subunit. CABYR, which is one of these four proteins, also interacts with a diverse array of signal tranducers via its SH3-, R2D2-, and proline-rich extension-like domains. AKAP3 and CABYR appear to associate in high molecular weight multi-protein complexes, which regulate the flagella' energy supply and movements. Diagonal gel electrophoresis experiments suggest that the high molecular weight signal-integrating scaffold partly is established by homo- and hetero-oligomerization of lower molecular weight splice variants of CABYR. The putative role of CABYR in lung cancer cells is finally discussed. |
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Authors:
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Søren Naaby-Hansen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Danish medical journal Volume: 59 ISSN: 2245-1919 ISO Abbreviation: Dan Med J Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-03-30 Completed Date: 2012-08-02 Revised Date: 2012-09-06 |
Medline Journal Info:
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Nlm Unique ID: 101576205 Medline TA: Dan Med J Country: Denmark |
Other Details:
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Languages: eng Pagination: B4414 Citation Subset: IM |
Affiliation:
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Department of Clinical Immunology, Aalborg Sygehus Nord, Aarhus University Hospital, 9000 Aalborg, Denmark. sonh@rn.dk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies
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immunology Autoimmunity / genetics, immunology Databases, Factual Electrophoresis, Gel, Pulsed-Field Glycosylphosphatidylinositols / immunology Humans Male Proteome / genetics, immunology* Spermatogenesis / genetics Spermatozoa / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Glycosylphosphatidylinositols; 0/Proteome |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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