| Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system. | |
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MedLine Citation:
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PMID: 19060383 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice. |
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Authors:
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Kwang-Wook Ko; Fengqiu Lin; Takumi Katsumata; Yoshinori Sugai; Sho Miyazaki; Hiroshi Kawaide; Kazunori Okada; Hideaki Nojiri; Hisakazu Yamane |
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Publication Detail:
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Type: Journal Article Date: 2008-12-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 72 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-12-24 Completed Date: 2009-02-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 3285-8 Citation Subset: IM |
Affiliation:
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Biotechnology Research Center, The University of Tokyo, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Cytochrome P-450 Enzyme System / metabolism* Gas Chromatography-Mass Spectrometry Gene Expression Microsomes / metabolism NADPH-Ferrihemoprotein Reductase / metabolism Oryza sativa / enzymology* Pichia / cytology, genetics* |
| Chemical | |
Reg. No./Substance:
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9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.12.-/ent-kaurene oxidase; EC 1.6.2.4/NADPH-Ferrihemoprotein Reductase |
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