Document Detail

Functional hyaluronan receptors are expressed on a squamous cell lung carcinoma cell line but not on other lung carcinoma cell lines.
MedLine Citation:
PMID:  7543820     Owner:  NLM     Status:  MEDLINE    
We investigated the production of hyaluronan and the presence of hyaluronan receptors in a panel of human lung carcinoma cell lines, consisting of small cell carcinomas (SCLC) and non-small cell carcinomas (non-SCLC). These transformed cell lines produced only minute amounts of hyaluronan, whereas normal lung fibroblasts synthesized high amounts. CD44 molecules (an integral membrane glycoprotein suggested previously to function as a hyaluronan receptor) were differentially expressed on non-SCLC cell lines but essentially not on the SCLC cell lines. In contrast, RHAMM molecules (receptor for hyaluronan-mediated motility) were preferentially expressed on SCLC cells. Although all the lung tumor cell lines expressed various amounts of CD44 and RHAMM, only the SCLC line U-1752 could bind [3H]hyaluronan. The binding sites were saturated with about 19,700 hyaluronan molecules (Mr 1.4 x 10(6)) bound per cell with a Kd of 0.16 x 10(-9) M. CD44 molecules were responsible for the binding activity since Hermes-1 antibodies that block the binding of hyaluronan to CD44 blocked the binding of [3H]hyaluronan to U-1752 cells. 4-Phorbol 12-myristate 13-acetate (PMA) treatment of U-1752 cells both increased the hyaluronan-binding activity in U-1752 cells as well as induced abrogation of cell-cell and cell-matrix interactions. Addition of hyaluronan inhibited the PMA-induced disassembly of the cells. The fact that CD44 molecules are able to bind [3H]hyaluronan only on the SCLC line U-1752 but not on other lung carcinoma cell lines may be of value as a marker for squamous cell carcinoma differentiation. Furthermore, the inhibitory effect of hyaluronan on the PMA-promoted cell disassembly suggest that hyaluronan surrounding squamous cell carcinoma cells may affect their migration and invasiveness.
P Teder; J Bergh; P Heldin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  55     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1995 Sep 
Date Detail:
Created Date:  1995-09-18     Completed Date:  1995-09-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3908-14     Citation Subset:  IM    
Department of Medical and Physiological Chemistry, Uppsala University, Sweden.
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MeSH Terms
Antigens, CD44
Carcinoma, Non-Small-Cell Lung / chemistry,  metabolism
Carcinoma, Small Cell / chemistry,  metabolism
Carcinoma, Squamous Cell / chemistry,  metabolism*,  pathology
Carrier Proteins / metabolism*
Culture Media, Conditioned / pharmacology
Fibroblasts / drug effects,  metabolism
Hyaluronic Acid / metabolism*,  pharmacology
Lung Neoplasms / chemistry,  metabolism*,  pathology
Neoplasm Proteins / metabolism*
Receptors, Cell Surface / metabolism*
Receptors, Lymphocyte Homing / metabolism*
Tetradecanoylphorbol Acetate / pharmacology
Tumor Cells, Cultured
Up-Regulation / drug effects
Reg. No./Substance:
0/Antigens, CD44; 0/Carrier Proteins; 0/Culture Media, Conditioned; 0/Neoplasm Proteins; 0/Receptors, Cell Surface; 0/Receptors, Lymphocyte Homing; 16561-29-8/Tetradecanoylphorbol Acetate; 9004-61-9/Hyaluronic Acid

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